Gut Microbiota, Humoral Immune Responses, Severity of Atopic Dermatitis, Skin Microbiota
Conditions
Keywords
atopic dermatitis, barrier function, gut, probiotics, skin
Brief summary
Hypothesis: Probiotics have been used as novel adjunct therapeutic approach in atopic dermatitis. In addition to balancing the gut microecology and promoting host immune defences, specific probiotics might further aid in controlling the microbial colonization of the skin, thereby reducing proneness to secondary infections which typically cause sustained symptoms. Thirty-nine infants with atopic dermatitis,randomized for a three-month-period in a double-blind design to receive extensively hydrolysed casein formula (NutramigenR, Mead-Johnson, USA) supplemented with (n=19) or without (n=20) Lactobacillus rhamnosus GG (ATCC 53103) 5.0 x 107 cfu/g to achieve a daily intake of 3.4 x 109 cfu. Sampling (blood and faecal samples, cotton swab from the skin) and clinical examination of the infant, including SCORAD assessment to determine the severity of atopic dermatitis, at each study visit (at entry and one month and three months thereafter).
Interventions
DIETARY_SUPPLEMENTCasein hydrolysate added with LGG
Infants drink extensively hydrolysed casein formula supplemented with LGG (ATCC 53103) 5.0 x 10 7 cfu/g to achieve a daily intake of 3.4 10 9 cfu.
DIETARY_SUPPLEMENTInfants drink casein hydrolysate without LGG
Infants drink extensively hydrolysed casein formula without added LGG
Sponsors
Academy of Finland
Mead Johnson Nutrition
Turku University Hospital
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
DOUBLE (Caregiver, Investigator)
Eligibility
Sex/Gender
ALL
Age
4 Weeks to 18 Months
Healthy volunteers
No
Inclusion criteria
* clinical diagnosis of atopic dermatitis * age 4 we - 18 mo
Exclusion criteria
* skin infection or severe infection at the time of enrollment
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| severity of atopic dermatitis | March 2007 - July 2008 | Severity of atopic dermatitis of the study infants will be assessed by SCORAD index |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Maturation of humoral immune responses | March 2007 - July 2008 | Determination of proportions of immunoglobulin secreting cells among peripheral blood mononuclear cells was carried out by ELISPOT assay. The proportions of CD 19+ memory B cells was carried out by flow cytometry |
Countries
Finland
Outcome results
None listed