Diabetes Mellitus, Type 2
Conditions
Brief summary
The purpose of this study is to study impairment of white blood cell function in patients with type II diabetes.
Detailed description
Leucocytes from poorly controlled diabetes exhibit aberrant chemotaxis, increased susceptibility to bacterial infection, leukotriene production, lysosomal enzyme release, proinflammatory cytokine expression and production of reactive oxygen species. Aberrant glucose concentration in diabetics affects functions of peripheral blood system as well as the immune system leading to impaired host defense. Impaired wound healing is a serious complication associated with diabetes. We hypothesized that impairment in leukocyte function results in dysfunctional inflammatory response in diabetic wounds. The proposed studies focus on characterizing mechanisms that will improve our understanding of the dysfunctional inflammatory response resulting in non-healing chronic wounds in diabetics.
Interventions
None listed
Sponsors
Sashwati Roy
Study design
Observational model
OTHER
Time perspective
OTHER
Eligibility
Sex/Gender
ALL
Age
40 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Adults ages 40-60 yrs old clinically diagnosed with Type II Diabetes * Adults ages 40-60 yrs old without Diabetes
Exclusion criteria
* Unable to provide informed consent * Pregnant Females * Therapeutically Immuno-compromised
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Ex vivo leukocyte function by measuring ROS production | immediately after blood draw | After blood draw monocytes are separated from whole blood and production of oxidants by these cells |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Ex vivo NADPH oxidase gene and protein expression | After blood draw | Gene and protein expressions are measured using Western blot and real time PCR. |
Countries
United States
Outcome results
None listed