Oxidative Stress, Single Nucleotide Polymorphism, Sleep Apnea Syndrome
Conditions
Keywords
NADPH oxidase
Brief summary
Obstructive Sleep Apnea (OSA) is associated with increased oxidative stress. The major sources of Reactive Oxygen Species (ROS) in the vasculature are the NADPH oxidases. Several polymorphisms related to NADPH oxidase expression or NADPH oxidase activity has been identified. The investigators are going to compare the distribution of the allelic frequencies of A-930G and C242T polymorphisms and their possible relationship with the levels of 8-isoprostanes as a marker of oxidative stress in patients with OSA and in a control group without OSA.
Detailed description
DESIGN: CASE-CONTROL STUDY METHODS: We are going to determine the A-930G and C242T p22phox genotypes in patients with OSA and healthy subjects recruited from sleep unit of Son Dureta University Hospital, (Palma de Mallorca, Spain). 8-Isoprostane is going to be measured as an oxidative stress marker with an 8-isoprostane EIA kit (Cayman Chemicals Company, USA) in plasma samples.
Interventions
None listed
Sponsors
Spanish Research Center for Respiratory Diseases
Sociedad Española de Neumología y Cirugía Torácica
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
Polyysomnografic study with Apnoea/Hypoapnoea Index higher to 10 in patients and lower in controls.
Exclusion criteria
* chronic obstructive pulmonary disease, liver cirrhosis, thyroid dysfunction, rheumatoid arthritis, chronic renal failure and/or psychiatric disorders.
Countries
Spain
Contacts
Primary ContactMónica de La Peña, MD
Outcome results
None listed