Leukemia
Conditions
Keywords
childhood acute myeloid leukemia/other myeloid malignancies
Brief summary
RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about biomarkers related to cancer. PURPOSE: This research study is studying biomarkers in tissue samples from young patients with acute myeloid leukemia.
Detailed description
OBJECTIVES: * Determine the frequency of ligand-independent phosphorylation of Stat3 in a small cohort of samples from pediatric patients with acute myeloid leukemia including, but not limited to, samples known to express mutated c-kit. * Measure the level of Stat3 phosphorylation in these samples after stimulation of three key cytokine receptors expressed on hematopoietic cells IL-6R, G-CSFR, and c-kit. OUTLINE: Cryopreserved samples are analyzed for levels of various components of the Stat3 pathway via western blotting.
Interventions
GENETICwestern blotting
OTHERlaboratory biomarker analysis
Sponsors
National Cancer Institute (NCI)
Children's Oncology Group
Study design
Observational model
CASE_ONLY
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
No minimum to 21 Years
Healthy volunteers
No
Inclusion criteria
DISEASE CHARACTERISTICS: * Diagnosis of acute myeloid leukemia * One of the following karyotypes: * t(8;21) and WT c-kit * t(8;21) and mutant c-kit * Normal karyotype PATIENT CHARACTERISTICS: * Not specified PRIOR CONCURRENT THERAPY: * Not specified
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Correlations between levels of pY-Stat3 and levels of upstream cytokine receptors | — |
| Association of constitutive and/or increased pY-Stat3 and c-kit genotype | — |
Outcome results
None listed