HIV Infections
Conditions
Keywords
pharmacogenomics, ATV, EFV, pharmacogenomics of ATV and EFV
Brief summary
Objectives: * To evaluate the impact of genetic polymorphism on ARV drug levels * To evaluate the effect of genetic polymorphism/drug levels on long term immunologic and virologic response * To correlate the genetic polymorphism/drug levels on antiretroviral toxicities The long-term objective of this research plan is to characterize impact of pharmacogenomics to HIV drug concentration, toxicities, and response to antiretroviral therapy among HIV-infected adults. A comprehensive understanding of the impact of pharmacogenomics to HIV infection and HIV medication will lead to the development of appropriate intervention such as dose reduction strategies in patients with particular gene(s) correlated with higher drug levels. The dose reduction strategy will decrease long term drug toxicity and cost saving for Thais and Asian Ethnicities.
Detailed description
The overall goal of this study is to characterize role of pharmacogenomic on ARV drug (atazanavir, and efavirenz) levels, its toxicities, and its long term efficacy among HIV-infected adults in Thailand. The specific aims are (1) to evaluate the impact of genetic polymorphism on ARV drug levels (2) to evaluate the effect of genetic polymorphism/ drug levels on long term immunologic and virologic response (3) to correlate the genetic polymorphism/drug levels on antiretroviral toxicities. The proposed study will be analysed in stored samples of the well-established cohort of long-term follow-up study for HIV-infected patients participated in HIV-NAT study protocols, the HIV-NAT006 study. This cohort provide us unique opportunity to study impact of pharmacogenomics on long term treatment response and long term drug toxicities since this cohort was started in 1996. Furthermore, the important factors include ARV regimen, drug toxicities, PBMC, immunological and virological parameters have been collected every 6 months basis in HIV-NAT006 study. A comprehensive understanding of the impact of pharmacogenomics to HIV infection and HIV medication will lead to development of appropriate intervention, particularly, dose reduction strategy in patient with particular gene correlated with greater drug levels. The dose reduction strategy will decrease long term drug toxicity and cost saving for Thai and Asian Ethnicity.
Interventions
None listed
Sponsors
Chulalongkorn University
Kirby Institute
Radboud University Medical Center
SEARCH Research Foundation
The HIV Netherlands Australia Thailand Research Collaboration
Study design
Observational model
COHORT
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
Inclusion criteria for pharmacogenomic of ATV: 1. On low-dose ATV/r at the time blood samples are collected for ATV drug levels 2. Age \> 18 years of age or older with HIV-1 infection 3. Provided consent form Inclusion criteria for pharmacogenomic of EFV: * On EFV at the time blood samples are collected for EFV levels * Age \> 18 years of age or older with HIV-1 infection * Patients who were on EFV but later switched to another ARV regimen due to toxicity of EFV and have stored sample at time of taking EFV * Provided consent form
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Frequency of MDR1-3435 allele variants,MDR1-2677 allele variants,UGT1A1 allele variants, frequency of CYP 2B6 variants in efavirenz treatment and compare candidate gene and treatment response of ATV/r or EFV | 2 years |
Secondary
| Measure | Time frame |
|---|---|
| Compare drug conc. of UGT1A1 variant with bilirubin, drug conc. & treatment resp. of ATV/r or EFV, drug conc.for WT, drug conc.for 2B6 variant with EFV toxicity & drug discontinuation, drug conc.or 2B6 variant with long term efficacy & EFV resistance | 2 years |
Countries
Thailand
Outcome results
None listed