Persistence of Infection With Helicobacter Pylori
Conditions
Keywords
helicobacter pylori, eradication failure
Brief summary
The immune response to Helicobacter pylori (Hp) importantly determines the pathogenesis of infection as well as the success of antibiotic eradication of the bacteria. The investigators want to demonstrate the importance of cathepsin X (CTSX), a cysteine protease, for the Hp eradication success. The diversity of the innate immune response to H. pylori antigens leading to either successful eradication of the infection or maintenance of chronic inflammation is connected to CTSX. The aim of this study is to determine whether H. pylori suppresses the CTSX expression and cytokine secretion in macrophage cell line THP-1 in the individuals that are not capable of eradicating the infection, opposite to H pylori in patients with successful H pylori eradication . The investigators also investigate the possibility whether strain-dependent differences in H. pylori lipopolysaccharide (LPS) influence the CTSX expression and cytokine secretion.
Interventions
DRUGclarithromycin, metronidazole, proton pump inhibitor
appropriate dose of antibiotics and proton pump inhibitor
Sponsors
University Medical Centre Ljubljana
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
No
Inclusion criteria
People with helicobacter gastritis and Helicobacter sensitive to antibiotic therapy but failure of therapy -
Exclusion criteria
People with helicobacter gastritis who did not eradicate Helicobacter pylori because of primary resistance to antibiotics. \-
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Evidence that cathepsin X influences on the eradication of Helicobacter pylori confirmed by lower expression of cathepsin X and cytokines in those patients. that can not eradicate Helicobacter pylori. | 7 months after last participant included in the study | The investigators assume that vast majority of patients, that have problems with eradication of Helicobater pylori, not caused by primary resistence to antibiotics, can not eradicate helicobacter because of inappropriate immune response. The investigators will measure cathepsin X (CTSX) expression and assume that those patients who have low concentrations of CTSX also have inappropriate immune response seen in low levels of cytokines. To treat such patients, you need to give them different and longer antibiotic therapy. |
Countries
Slovenia
Contacts
Primary ContactAlojz Ihan, MD, PhD
Backup ContactMiha Skvarc, MD
Outcome results
None listed