Metabolism
Conditions
Keywords
Fructose, VLDL-triglycerides, de novo lipogenesis, Human metabolism
Brief summary
8 healthy male volunteers will be studied each on 3 occasions, ie * after 4 days on a weight maintenance diet containing 5% simple sugars + low physical activity * after 4 days on a weight maintenance diet containing 30% fructose + low physical activity * after 4 days on a weight maintenance diet containing 30% fructose + high physical activity At the end of each of these 3 periods, fructose induced hepatic de novo lipogenesis (13C palmitate synthesis in response to ingestion of a 13C fructose load) and plasma VLDL-triglyceride kinetics (measured with a bolus of 2H-labeled glycerol) will be measured
Interventions
BEHAVIORALweight maintenance, low sugar diet
BEHAVIORALweight maintenance, high fructose diet
BEHAVIORALweight maintenance, high fructose diet+ exercise
Sponsors
Centre Hospitalier Universitaire Vaudois
University of Lausanne
Study design
Allocation
RANDOMIZED
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* age between 18 and 30 years * males * BMI between 19 and 25 * low to moderate physical activity (\< 3 sessions/week)
Exclusion criteria
* smoking * consumption of alcohol\>50g/week * consumption of drugs or illicit substances * food allergies
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Hepatic de novo lipogenesis | after 4 days on controlled diet+exercise | Measurement of 13C VLDL palmitate after ingestion of 13C fructose |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Plasma VLDL-triglyceride kinetics | after 4 days on controlled diet+exercise | Modelling of 2H5-glycerol incorporation in VLDL afetr administration of a bolus of 2H5 glycerol |
Countries
Switzerland
Outcome results
None listed