Ischemic Preconditioning
Conditions
Keywords
ischemic preconditioning,serum proteins,human
Brief summary
Numerous studies Have shown that limb ischemic preconditioning can protect vital organs (including the heart) from ischemia-reperfusion injury,which has a broad application prospect.But its mechanism is still unclear. Evidence showed that humoral mechanisms may play an important role. This study was carried out on the limb ischemic preconditioning in healthy volunteers, collected their serum at different time points before and after treatment,classified and identified the serum proteins during different periods of limb ischemic preconditioning,by using methods including high abundant protein removal,Two-dimensional electrophoresis chromatography and mass spectrometry,then analysed activation and synthesis of the proteins in order to search for the proteins or peptides whose synthesis was activated by ischemic preconditioning. This study will be the first systemic explore of the changes in serum proteins of limb ischemic preconditioning in human body,and lay theoretical basis for the clinical application of limb ischemic preconditioning and for further explore of its humoral mechanism,thus provide clues for searching for protein or peptide having protective effects for organs.
Detailed description
We aim to assess whether limb ischemic preconditioning protects remote tissue or organs through a humoral mechanism.
Interventions
PROCEDURElimb ischemia preconditioning
limb ischemic preconditioning consists of three 5-min cycles of left upper limb ischemia induced by a blood pressure cuff placed on the left upper arm and inflated to 200 mmHg,with an intervention 5-min of reperfusion during which the cuff was deflated.
Sponsors
Sun Yat-sen University
Study design
Allocation
RANDOMIZED
Intervention model
SINGLE_GROUP
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
20 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy volunteers aging 20-30 years old * no cardiac，hepatic or renal，peripheral vascular diseases * be willing to enter our study and sign an written informed consent
Exclusion criteria
* younger than 20 years old or older than 30 years old * having cardiac，hepatic or renal，peripheral vascular diseases * refuse to enter our study or sign an written informed consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Transient limb ischemia would change expression of several serum proteins in humans,indicating a humoral mechanism for its organ protection. | two years | Proteomics analysis of the serum proteins. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| A questionnaire study of the acceptability of transient limb ischemia in healthy conscious subjects. | one year | Transient limb ischemia as a remote ischemic preconditioning stimulus has been shown an effective and non-invasive strategy to protect remote tissue or organs from an extended ischemic event. But the acceptability of human subjects remains unknown. Our study aims to assess the acceptability of this transient limb ischemia protocol in healthy conscious subjects. |
Countries
China
Outcome results
None listed