the Effect of MTHFR C677T on Folate Metabolism
Conditions
Keywords
Folate, MTHFR, Homocysteine
Brief summary
The common polymorphism in MTHFR gene (C677T) has a significant effect on (6S)-5-CH3-H4folate after folic acid supplementation. For example, post supplementation differences in (6S)-5-CH3-H4folate between CC and TT are above 30%.
Detailed description
The investigators aim to test the effect of low doses of folic acid with or without B6 and B12 on folate forms in relation to polymorphisms in folate catabolising enzymes in elderly people. Our hypothesis is that differences in folate forms after folic acid supplementation might be due to polymorphisms in folate catabolising enzymes. Therefore, folic acid might be retained by people with certain genotypes.
Interventions
DIETARY_SUPPLEMENTfolic acid plus B6 and B12
folic acid 400 µg plus vitamin B12 plus B6
DIETARY_SUPPLEMENTfolic acid
folic acid 400 µg
Sponsors
Universität des Saarlandes
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
65 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Males and females * Age \> 65 years.
Exclusion criteria
* Renal dysfunction * Recent stroke, or coronary event (within the last 3 months) * Current cancer treatment * Antifolate (e.g. methotrexate) treatment * Ileum resection * Current B-vitamin supplement * Epilepsy medications * Megaloblastic anemia or other indications for treatment with high doses of folate or vitamin B12.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| blood metabolic markers | 18 months | we will measure primary folate forms after folic acid supplement |
Countries
Germany
Outcome results
None listed