Chronic Obstructive Pulmonary Disease
Conditions
Keywords
macrophages, alveolar, monocytes, Receptor Protein-Tyrosine Kinases, Humans, Phagocytosis, Lung, in vitro
Brief summary
The purpose of this study is to compare the ability of two types of white blood cells to eat dead host cells, and how this process affects their ability to protect the body from infection. The two cell types are monocytes, a cell in the bloodstream, and alveolar macrophages, a cell in the lung that is ultimately derived from monocytes
Detailed description
Volunteers, who may be Veterans or non-Veterans, will be screened by a series of questionnaires, chest X-ray, spirometry, EKG, blood work, and a complete history and physician examination by a study physician. Those who qualify for the study and agree via informed consent, will undergo a fiberoptic bronchoscopy on a separate day for the initial visit. Bronchoscopy is a procedure in which a flexible video instrument is passed via the nose or mouth, through the vocal cords and into the lungs. During the procedure, portions of the lungs will be washed (lavaged) with a salt water solution, to collect the alveolar macrophages. This procedure involves conscious sedation, so that a driver is required on the day of the bronchoscopy. The entire procedure, including placement of an intravenous line to administer medications, local anesthetics, the bronchoscopy itself, and the recovery period, takes about 2.5-3 hours. The cells collected by bronchoscopy will be studied in the research laboratory, and used entirely in that procedure.
Interventions
PROCEDUREFiberoptic bronchoscopy
which a flexible video instrument is passed via the nose or mouth, through the vocal cords and into the lungs. During the procedure, portions of the lungs will be washed (lavaged) with a salt water solution, to collect the alveolar macrophages. This procedure involves conscious sedation, so that a driver is required on the day of the bronchoscopy. The entire procedure, including placement of an intravenous line to administer medications, local anesthetics, the bronchoscopy itself, and the recovery period, takes about 2.5-3 hours.
PROCEDUREblood draw
Blood will be collected at the enrollment visit to test the subject to exclude disorders that would make it unsafe to perform the bronchoscopy, including severe anemia, infection, or disorders of kidney or liver function.
PROCEDUREintravenous catheter
An intravenous catheter is placed on the day of the bronchoscopy to permit administration of sedative medications.
Sponsors
VA Office of Research and Development
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
* Veteran or non-Veteran * Able to give informed consent
Exclusion criteria
* Unstable heart disease * Other systemic disease and unlikely to survive at least 2 years * Mental incompetence * Prednisone \>20 mg/day * Participation in another interventional protocol within last 6 weeks * Asthma * Cystic fibrosis * Clinically significant bronchiectasis * Lung cancer or any cancer not in remission for at least 5 years * Other inflammatory or fibrotic lung disease * Use of antibiotics for a lung infection within the past 4 weeks * Autoimmune disease, i.e., Rheumatoid arthritis, systemic lupus erythematosus, autoimmune hepatitis, Crohn's disease or other
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| macrophage uptake of apoptotic cells in vitro | 1-7 days |
Secondary
| Measure | Time frame |
|---|---|
| spirometric measurement of lung function | 1-30 days |
Countries
United States
Outcome results
None listed