Insulin Sensitivity
Conditions
Brief summary
The investigators aimed to evaluate the health benefits of physical training per se, weight loss per se and exercise-induced weight loss in young, overweight men. The investigators hypothesized that physical training would have independent effects on various health parameters (insulin sensitivity, fasting glucose and insulin, total and abdominal fat mass, waist circumference, blood pressure and key muscle proteins), and that a concomitant weight loss from physical activity would increase the effect.
Detailed description
At baseline, during the intervention and in the week following the intervention participants underwent a panel of tests: * Cardio respiratory fitness * blood pressure * anthropometric measures At baseline and after the intervention a hyperinsulinemic, euglycemic clamp were performed to asses insulin sensitivity. Tissue and blood samples were also collected for further analysis: * Total-protein analysis of selected proteins * Fasting plasma glucose, insulin and free fatty acids
Interventions
BEHAVIORAL12 weeks of lifestyle change
Participants were randomly assigned to a 12-week intervention in one of four groups: Training (T), energy-reduced Diet (D), Training and increased- Diet (T-iD) or Control (C). The interventions consisted of: T: increased physical activity through daily endurance training equivalent to 600 kcal/day, unchanged diet; D: sedentary lifestyle, reduced diet by 600 kcal/day primarily through reducing serving sizes and substituting energy dense with less energy dense nutrients; T-iD: increased physical activity through daily endurance training equivalent to 600 kcal/day, increased diet by 600 kcal/day primarily through increasing serving sizes and consuming energy dense nutrients; C: sedentary lifestyle and unchanged diet.
Sponsors
University of Copenhagen
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
20 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Overweight (body mass index 25-30 kg/m2) * Weight stable (±2 kg during the past 6 months) * Body fat% \>25%, * Maximal oxygen uptake \<45 mL/min/kg * Sedentary and healthy according to interview * Fasting glucose \<6.1 mmol/L * Blood pressure \<140/90 mmHg * No first degree relatives with type-2-diabetes
Exclusion criteria
* Resistance training * Inability to meet intervention criteria
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in insulin sensitivity | week 0 and 13 | Before and after the 12 week interventions a 1-step, 120 min, hyperinsulinaemic, euglycemic clamp was performed. After an initial 2mL bolus of insulin infusate, insulin was infused at 40 microU/min/kg. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changes in body composition | DEXA: week 0, 2, 6, 10, and 13; MRI: Week 0 and 13 | Before, during and after the 12-week interventions changes in: Body mass, Lean body mass, Fat mass, Abdominal fat mass, and Viceral and subcutaneous fat were assesed by DEXA and MRI scans. |
Countries
Denmark
Outcome results
None listed