Stroke
Conditions
Keywords
stroke-induced immunodepression, acute media infarct, acute intracerebral bleeding, leucocytes, autoaggressive T-cells, acute media infarct or acute intracerebral bleeding
Brief summary
The damage of the brain parenchyma, as well as the stroke-induced dysfunction of the blood-brain-barrier can make previously hidden CNS antigens visible, and can thus lead to the development of autoimmune mechanisms. It seems plausible that stroke-associated immunodepression influences the development and the phenotype of these autoreactive immune responses. This study will investigate whether cerebral ischemia leads to changes in the immune response, in particular to the development and/or proliferation of autoreactive effector T-cells and/or regulatory T-cells. Furthermore, the association between the severity and the phenotype of this autoimmune response and the clinical course, i.e. prognosis and mortality, will be investigated.
Interventions
None listed
Sponsors
NeuroCure Clinical Research Center, Charite, Berlin
Charite University, Berlin, Germany
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* acute media infarct or intracerebral bleeding within the last 36 h (patients) * NIHSS \> 7 (patients) * age \> 17 years (patients), age \> 54 years (controls) * informed consent of patient or legal representative/ of control * cardiovascular risk such as diabetes mellitus (control)
Exclusion criteria
* infections (patients, controls) * antibiotic or immunosuppressive treatment within the last 4 weeks (patients) * other CNS disorders
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| autoantigen-specific T-cells in patients with acute media infarct | within 36 h | quantitative determination of autoantigen-specific T-cells in patients with acute media infarct |
| leukocytes in patients with acute media infarct | within 36 hours | quantitative and qualitative analysis of leukocytes in patients with acute media infarct |
Secondary
| Measure | Time frame |
|---|---|
| frequency and phenotype of CNS-autoreactive immune cells under the influence of immunodepression | within 36 h, after day 3, 7, 90 and 180 |
| clinical course, i.e. mortality and prognosis (measured by mod. Rankin Scale) | after day 90 and 180 |
| clinical course, i.e. mortality and prognosis (measured by Bartel Index) | after day 90 and 180 |
Countries
Germany
Outcome results
None listed