Healthy
Conditions
Keywords
L-NMMA, blood pressure, arterial stiffness, pulse wave velocity, augmentation index, dose-response, healthy subjects, GFR
Brief summary
The purpose of this study is to investigate the effect of different levels of nitric oxide inhibition on blood pressure and arterial stiffness in healthy subjects. L-NMMA, an inhibitor of the nitric oxide synthase, is administered as bolus injection followed by continuous infusion, and is expected to raise blood pressure and arterial stiffness according to L-NMMA dose.
Interventions
3 mg/kg IV priming dose + 2 mg/kg/hr IV infusion for 1 hour
DRUGPlacebo
Isotonic saline solution IV priming dose + IV infusion for 1 hour
Sponsors
Regional Hospital Holstebro
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy males * Age 18-35 years * BMI 18.5-30.0
Exclusion criteria
* Tobacco or illicit drug use * Current use of any prescription or non-prescription drugs * Alcohol abuse * History of heart failure, lever failure, chronic kidney disease or cerebral insult * Diabetes * Clinical or paraclinical signs of infection * Pathologic ECG * Ambulatory blood pressure \>140/90 mmHg * Abnormal blood or urin screening tests
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Systolic blood pressure | 6 hours |
Secondary
| Measure | Time frame |
|---|---|
| Arterial Stiffness | 6 hours |
| Fractional excretion of sodium | 6 hours |
| Fractional excretion of potassium | 6 hours |
| GFR | 6 hours |
| Plasma angiotensin II | 6 hours |
| Plasma aldosterone | 6 hour |
| Plasma renin concentration | 6 hour |
Countries
Denmark
Outcome results
None listed