Neuroendocrine Reproductive Status
Conditions
Keywords
LH pulsatility, GnRH regulation, isocaloric nutrition, hypercaloric nutrition, euglycemic clamps, hyperinsulinemic clamps, testosterone, LH, leptine, ghrelin, PYY, CCK, GLP-1, GIP, cortisol
Brief summary
This study will evaluate the potential effects of a changing metabolic environment on GnRH hypothalamic neurons, by performing studies of LH pulsatility (basal and during clamp studies) in different groups of male subjects fed isocaloric and hypercaloric diets. The participation of other gastro-intestinal tract hormones to these regulations will be systematically evaluated.
Interventions
DIETARY_SUPPLEMENTeffect of metabolic variations on LH pulsatility
Isocaloric diet: 1,5 times basal metabolism. Constitution:55% carbohydrates, 15% proteins and 30% fat. Hypercaloric diet: addition of 30% fat and 3 g of fructose/kg of weight to isocaloric diet. PO and IV glucose during clamps: PO administration of 90 mg/kg glucose at 90 minutes intervals during the clamp, and adjustment of the IV perfusion to keep euglycemia.
Sponsors
Centre Hospitalier Universitaire Vaudois
Study design
Allocation
NON_RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 25 Years
Healthy volunteers
Yes
Inclusion criteria
* male * age between 18 and 25 years old * no known pathology * normal physical exam * BMI between 20 and 25 kg/m2
Exclusion criteria
* history of endocrine disease * no known infertility (no conception after 12 months of regular unprotected intercourse) * abnormal results on the initial blood tests (CBC, fasting glucose and insulin, HbA1C, TSH, free T4, LH, FSH, inhibin B and total testosterone)
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Luteinizing hormone (LH) pulsatility | 2 years |
Countries
Switzerland
Outcome results
None listed