Tissue Sectioning by Electro-Dissociation

Status

Withdrawn

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT01054612

Enrollment

Registered

2010-01-22

Start date

2002-03-31

Completion date

2010-01-31

Last updated

2015-03-04

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Fresh Unfixed Tissues, Fresh Unfrozen Tissues, Thin Sections

Keywords

Histology, Immunohistochemical, Gene Expression, Tissue Sectioning

Brief summary

Currently there is no technique to produce thin (0.004-0.01 mm) serial sections of large fresh tissue specimens that are suitable for high-resolution in situ protein/gene expression studies without ice artifact or fixation-induced molecular damage. Traditional frozen sectioning preserves protein and nucleic acid structure, but the inherent ice artifact precludes reconstruction of protein and mRNA expression patterns in 3-dimensions. Since the limitations of the existing sectioning techniques result from the fact that they rely on mechanical cutting which in turn require the tissue to be stiff, we suggest a new approach to cut tissue via an electro erosion process that utilizes focus radio frequency (RF).

Interventions

OTHERTissue Sectioning via Electro Erosion Process

Focused RF energy can be used to produce consecutive thin sections of fresh tissue for immunohistochemical and nucleic acids analysis by electro-dissociation without thermal damage, ultimately allowing high resolution reconstruction of gene and protein expression patterns of large tissue specimens in 3D.

Eligibility

Sex/Gender

ALL

Healthy volunteers

Inclusion criteria

* Fresh Tissue * Unfrozen Tissue * Unfixed Tissue

Exclusion criteria

* Unfresh Tissue * Frozen Tissue * Fixed Tissue

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026