Cardiovascular Status, Hepatic Fatty Acid Metabolism, Oxidative Stress, Systemic Insulin Resistance
Conditions
Keywords
fructose, fatty liver disease, diet
Brief summary
Dietary consumption of fructose has increased by nearly 50% since 1960. A high fructose diet (HFrD) results in greater visceral adiposity and systemic insulin resistance than a high glucose diet. The effects of fructose on liver fatty acid and ATP stores, systemic oxidative stress and cardiovascular status are not fully known.
Detailed description
The protocol will assess the following outcomes: 1. The ultimate fate of this increased hepatic fatty acid production following a high fructose vs. glucose diet 2. The effect of a high fructose vs. glucose diet on liver ATP stores 3. The effect of a high fructose vs. glucose diet on markers of oxidative stress 4. The effect of a high fructose vs. glucose diet on cardiovascular status Factors critical to carbohydrate metabolism such as systemic insulin resistance, body composition, energy expenditure, physical activity will also be assessed. 32 centrally overweight healthy males with a low baseline fructose intake will be recruited. They will be randomised double blindly to receive 25% of their dietary energy requirements from either fructose or glucose for 14 days. The sugars will first be taken in an energy balanced and then an overfeeding setting.
Interventions
DIETARY_SUPPLEMENThigh sugar diet
25% of dietary energy from fructose or glucose
Sponsors
University of Nottingham
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
1. Body mass index 25-32 2. Waist \> hip circumference 3. Age 18-50 years 4. Male
Exclusion criteria
1. Reported weight change \> 3 kg in prior 3/12 2. Active health problems 3. Contraindications to MRI scanning 4. Symptoms of functional bloating or irritable bowel syndrome 5. Abnormal liver or renal function tests 6. Random glucose greater than 11.0 mmol/L 7. Evidence of metabolic or viral liver disease as screened for by hepatitis B and C serology, and ferritin. 8. Alcohol intake \> 21 units per week 9. Vegetarianism 10. Normal daily fructose intake from drinks greater than that in 500ml of coca cola 11. Abnormal carbohydrate energy contribution to baseline diet - defined as greater than 2 standard deviations from the mean of the National Diet and Nutrition Survey 2002 data
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Liver triglyceride content | 2 weeks |
Secondary
| Measure | Time frame |
|---|---|
| Total abdominal visceral fat content (MRI) | 2 weeks |
| Liver ATP, PME concentration and pH (31P MRS) | 2 weeks |
| Liver de novo lipogenesis | 2 weeks |
| Muscle triglyceride content | 2 weeks |
| Hepatic and systemic insulin resistance | 2 weeks |
| Cardiovascular measures using finometry | 2 weeks |
| Systemic oxidative stress and inflammatory cytokine profile | 2 weeks |
| Resting energy expenditure, lipid and carbohydrate oxidation rates | 2 weeks |
Countries
United Kingdom
Outcome results
None listed