Abnormal Expression Proteins, Mitochondrial DNA and miRNA of Irritable Bowel Syndrome

Screening and Identification of Abnormal Expression Proteins and miRNA From Colonic Mucosa of Patients With Irritable Bowel Syndrome.

Status

UNKNOWN

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT01028898

Acronym

IBS

Enrollment

180

Registered

2009-12-09

Start date

2006-01-31

Completion date

2012-12-31

Last updated

2011-02-11

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Colonic Diseases, Colonic Diseases, Functional, Digestive System Diseases, Gastrointestinal Diseases, Intestinal Diseases, Irritable Bowel Syndrome

Keywords

IBS, proteomics, fluorescent quantitation polymerase chain reaction, Western blot, immunohistochemistry staining (IHC), ATP content, micRNA

Brief summary

In the investigators study, the investigators will focus on the screening of the related proteins and miRNA to IBS in order to reveal the possible clues or molecular mechanism for this disorder.

Detailed description

For the proteomic procedures, Our study groups extracted proteins from the cecum and sigmoid colon samples and ran on the two-dimensional gel electrophoresis (2-DE). The proteins on 2-DE were visualized by the silver stain, and then gel maps were scanned and analyzed with the Image Master 2D Elite. The obvious abnormal protein spots were identified by the mass spectrometry. Western blot 、 immunohistochemistry 、 mRNA and miRNA were also performed for further verification of the proteins.

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to 60 Years

Healthy volunteers

Yes

Inclusion criteria

* Patients with symptoms meeting Rome III criteria for diagnosis of IBS-C and IBS-D

Exclusion criteria

* Patients with organic illnesses or a history of major abdominal surgery * Major psychotic episode, mental retardation or dementia * Pregnant women and lactational women * Postinfectious IBS, PI-IBS

Outcome results