Infertility
Conditions
Keywords
Embryo culture, IVF, Microfluidics, infertility
Brief summary
Embryo culture is a cornerstone of in vitro fertilization treatments. Usually, embryos are cultured in microdrops of culture media in incubators with controlled temperature, humidity and atmosphere. However, these culture systems are static, while in vivo the initial growth of the embryo takes place in the uterine tube and they are submitted to a dynamic environment. The aim of this study is to compare the embryo development on days 2 and 3 of culture morphological features of embryos under static and dynamic(microfluidics) systems of culture.
Detailed description
Embryo culture is a cornerstone of in vitro fertilization treatments. Usually, embryos are cultured in microdrops of culture media in incubators with controlled temperature, humidity and atmosphere. However, these culture systems are static, while in vivo the initial growth of the embryo takes place in the uterine tube and they are submitted to a dynamic environment. The aim of this study is to compare the embryo development on days 2 and 3 of culture morphological features of embryos under static and dynamic(microfluidics) systems of culture.
Interventions
DEVICESMART dynamic system of embryo culture
Embryos will be cultured in a dynamic system of microfluidics.
DEVICEStatic culture
Embryos cultured in 40 microliters microdrops of G-IVF plus series V
Sponsors
Incept Biosystems
Federal University of São Paulo
Study design
Allocation
RANDOMIZED
Intervention model
SINGLE_GROUP
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
21 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Age between 21 and 35 years old * No more than 1 previous IVF failure * Absence of physical conditions that can interfere in oocyte quality * At least 8 zigotes 24 hours after fertilization
Exclusion criteria
* Use of epididimary or testicular sperm * Sperm or egg donation * Untreated systemic diseases
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Embryo morphology on days 2 and 3 of embryo culture according to the criteria described by Lucinda Veeck | Embryo culture for 3 days before transference to the uterus |
Secondary
| Measure | Time frame |
|---|---|
| Implantation rate and pregnancy rate | 24 month |
Countries
Brazil
Contacts
Primary ContactJose R Alegretti, BSc
Backup ContactAndre M Rocha, PhD
Outcome results
None listed