Macular Disease
Conditions
Keywords
electron microscopy, internal limiting membrane, macular disease, optical coherence tomography
Brief summary
this study is to analyze and compare the ultrastructure of the retinal and vitreous plane of the epiretinal membrane and internal limiting membrane removed during macular surgery and to evaluate relation between the ultrastructure of the epiretinal membrane and internal limiting membrane and surgical outcomes.
Detailed description
prospective, interventional study and includes patients who underwent vitrectomy,peeling of an epiretinal membrane and internal limiting membrane excision with or without intraoperative intraocular ICG injection. Excised specimens were placed on a Millipore filter, fixed in a paraformaldehyde solution, and examined by light and transmission electron microscopy. Ultrathin sections were stained with uranyl acetate-lead citrate and inspected in a Zeiss EM electron microscope. The assessment was done in a blinded, masked fashion so that the clinicians who reviewed the histologic specimens
Interventions
PROCEDUREvitrectomy for macular disease
three-port pars plana vitrectomy was performed in each case using a vitreous cutter surrounded by a coaxial optic fiber connected to a xenon light source (Lausanne set, Oertli, Switzerland-developed at Jules Gonin, Lausanne, by Gonvers and Bovey). Separation of the posterior hyaloid membrane was performed when necessary. Visualization of the fundus was achieved with a special noncontact wide-angle viewing system 37 during vitrectomy and with a planoconcave contact lens for macular peeling. The MEM was peeled in the macular area using an end gripping forceps.
Sponsors
Hallym University Medical Center
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
20 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* patients who underwent vitrectomy,peeling of an idiopathic MEM and ILM excision with or without intraoperative intraocular ICG injectio
Exclusion criteria
* the presence of macular hole or lamellar macular hole previous vitreoretinal surgery the presence of any other macular pathologic features potentially interfering with histologic results (such as diabetic retinopathy or age-related macular degeneration).
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| spectral domain optical coherence tomography | baseline, 1week, 1month, 3 month | The central 1mm subfield thickness the average macular thickness the inner nuclear layer thickness outer nuclear layer thickness outer plexiform layer thickness photoreceptor integrity ILM map RPE map |
| best corrected visual acuity | baseline, 1 month, 3 months | logMAR |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| electron microscopy finding of internal limiting membrane | during operation | — |
| visual field | baseline, postoperative 1 months, 3 months | humpley visual field |
| preferential hyperacuity perimeter | baseline, postoperative 1 months, 3 months | ForeseePHP 2.05; Notal Vision, Tel Aviv, Israel |
Countries
South Korea
Outcome results
None listed