Healthy
Conditions
Brief summary
Alcohol has consequences including increased risk for upper respiratory tract infections, pneumonia, acute respiratory distress syndrome (ARDS), and alcohol-induced asthma. The investigators have established that airways are specifically impacted by alcohol exposure because the airways are heavily exposed to the vapor phase of alcohol during drinking. These preliminary studies demonstrate that brief alcohol administration significantly attenuates airway hyperresponsiveness (AHR) in a mouse model leading to the hypothesis that alcohol exposure modifies airway hyperresponsiveness through a cAMP/NO- dependent mechanism.
Detailed description
Alcohol has well-established consequences in the lung including increased risk for upper respiratory tract infections, pneumonia and acute respiratory distress syndrome (ARDS). There have even been a few reports of alcohol-induced asthma. Data from the investigators' laboratory have established that the airways are specifically impacted by alcohol exposure. Because the airways are heavily exposed to the vapor phase of alcohol during drinking and airway motor tone is modulated by cAMP, the investigators speculated that airway bronchial motor function would be altered in mice fed alcohol. The investigators' preliminary studies demonstrate that brief alcohol administration significantly attenuates airway hyperresponsiveness (AHR) in a mouse model. This novel finding has led us to hypothesize that: Alcohol exposure modifies airway hyperresponsiveness through a cAMP/NO- dependent mechanism.
Interventions
OTHERethanol
subjects will ingest 3 ounces of vodka mixed with fruit juice within 30 min.
Sponsors
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
University of Nebraska
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
21 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* male * must be of legal drinking age in the state of Nebraska (≥ 21) * be between the ages of 21-65 * be non-smokers * be able to dedicate 3-4 hours on two consecutive days (including waiting at least 2 hours after the alcohol ingestion) * able to provide informed consent
Exclusion criteria
* female * inability to give informed consent * any history of lung or allergic disease * any alcohol intake for the week prior to the experiment * self-identified history of chronic heavy drinking or alcoholism or psychiatric disorder * If an otherwise qualifying participant has previously undocumented or unidentified asthma as indicated by the baseline methacholine challenge, that subject will be excluded from the remainder of the study and replaced by another subject
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in airway hyperresponsiveness. | 2 hours | A one-half concentration difference in the PC20FEV1 will be considered a statistically significant change in airway hyperresponsiveness. |
Countries
United States
Outcome results
None listed