Microtia
Conditions
Keywords
tissue engineering
Brief summary
Platelet-rich plasma (PRP) has mixed growth factors such as TGF-ß1 and TGF-ß2, vascular epithelial growth factor (VEGF), platelet-derived growth factor (PDGF), and insulin-like growth factor (IGF). These growth factors appear to play an important role in wound healing and are assumed as promoters of tissue regeneration. Moreover, PRP was used as injectable scaffold seeded with chondrocytes to regenerate cartilage. In their previous study, the investigators concluded that growth factors in PRP can effectively react as a growth factor cocktail to induce human nucleus pulposus proliferation and differentiation, and also promote tissue-engineered nucleus pulposus formation. The investigators also have a hypothesis that PRP can promote tissue-engineered microtia auricular cartilage formation. TGF- ß1 exists in the highest concentration and is more important among all of the growth factors released from PRP. So TGF- ß1 can be used as the core ingredient and the indicator for applying PRP in these studies. The aim of this study was to compare the histological and biochemical character of microtia chondrocytes treated with and without PRP.
Interventions
PRP was extracted from total blood and TGF-b1 was used as indicator
OTHERChondrocyte culture with FBS medium
Microtia chondrocyte culture with FBS medium only
Sponsors
Taipei Medical University WanFang Hospital
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* microtia patient
Exclusion criteria
* non microtia patient
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The aim of this study was to compare the histological and biochemical character of microtia chondrocytes treated with and without PRP. | 4 weeks |
Countries
Taiwan
Outcome results
None listed