Metabolome and Microbiomic in Fecal Samples

Fecal Samples for Metabolomic and Microbiomic Studies: Effects of Preparation and Storage

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT00938028

Acronym

MicroMet

Enrollment

Registered

2009-07-13

Start date

2009-04-30

Completion date

2009-05-31

Last updated

2009-07-21

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Infant

Keywords

microbiome, metabolome, stool, fecal samples, handling, toddler

Brief summary

The purpose of this study is to evaluate the effects of storage and handling on fecal samples collected for metabolomic, proteomic and microbiomic and analysis.

Detailed description

Background Human fecal collection for clinical or research studies usually occurs at the residence of the subject, an environment not equipped with measures to accommodate proper preparation and storage of samples for microbiome and metabolome analysis. Aims To evaluate the effects of storage and handling on fecal samples collected for metabolomic, proteomic and microbiomic and analysis. Methods Fecal samples will be collected within several minutes of voiding from healthy infants at the time of diaper change at day-care center. Samples will be divided into 7 tubes, and subjected to different storage temperatures, before preforming microbial analysis by DNA sequencing, and metabolome analysis by H1NMR spectroscoty.

Interventions

None listed

Study design

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

12 Months to 20 Months

Healthy volunteers

Yes

Inclusion criteria

* healthy * age 12-20 months

Design outcomes

Primary

Measure	Time frame
Microbiome and metabolome yield	one month

Countries

United States

Outcome results