Breast Cancer, Chronic Myeloproliferative Disorders, Gestational Trophoblastic Tumor, Graft Versus Host Disease, Leukemia, Lymphoma, Multiple Myeloma and Plasma Cell Neoplasm, Myelodysplastic/Myeloproliferative Neoplasms, Myelodysplastic Syndromes, Neuroblastoma, Ovarian Cancer, Testicular Germ Cell Tumor
Conditions
Brief summary
RATIONALE: Collecting and storing samples of blood, urine, and tissue from patients undergoing a donor stem cell transplant to test in the laboratory may help the study of graft-versus-host disease in the future. PURPOSE: This research study is collecting and storing tissue and DNA samples from patients undergoing a donor stem cell transplant.
Detailed description
OBJECTIVES: * To establish a DNA bank from donor and recipient (prior to transplant) peripheral blood for SNP studies. * To establish a tissue bank for gene expression studies using donor and recipient (post-transplant) peripheral blood samples. * To establish a tissue bank for proteomic studies using donor and recipient blood and urine samples. * To establish a tissue bank for various studies using biopsy specimens. * To establish a tissue bank for flow cytometric studies using recipient (post-transplant), and sample from stem cell product. OUTLINE: Peripheral blood samples are collected from donors prior to stem cell mobilization and from patients prior to starting preparative regimen. Samples are studied by single nucleotide polymorphism (SNP) analysis, SNP array, proteomic analysis, gene expression, and other immunological laboratory methods. Blood, urine, and tissue samples are preserved for future studies. Extra skin biopsies and gastrointestinal biopsies are performed at diagnosis of graft-vs-host disease and preserved in a tissue bank for flow cytometric studies.
Interventions
GENETICgene expression analysis
blood collection
GENETICmicroarray analysis
Blood collection
GENETICpolymorphism analysis
Blood collection
GENETICproteomic profiling
Blood collection
OTHERflow cytometry
Blood collection
OTHERimmunologic technique
Blood collection
OTHERlaboratory biomarker analysis
Blood collection
PROCEDUREbiopsy
Blood collection
Sponsors
National Cancer Institute (NCI)
Vanderbilt-Ingram Cancer Center
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
DISEASE CHARACTERISTICS: * Approved for allogeneic stem cell transplantation * Sample of the stem cells from donor must meet 1 of the following criteria: * Total peripheral blood stem cell dose \> 5 X10\^6 CD34+ cells/kg * Marrow cell dose \> 3 X 10\^8 nucleated cells/kg PATIENT CHARACTERISTICS: * Platelet count \> 50,000/mm³ (for patients undergoing endoscopic biopsies) * INR \< 1.5 (for patients undergoing endoscopic biopsies) PRIOR CONCURRENT THERAPY: * See Disease Characteristics
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| DNA bank from donor and recipient (prior to transplant) peripheral blood for single nucleotide polymorphism studies | Completion of transplant |
| Tissue bank for gene expression studies using donor and recipient (post-transplant) peripheral blood samples | End of treatment |
| Tissue bank for proteomic studies using donor and recipient blood and urine samples | End of treatment |
| Tissue bank for flow cytometric studies using recipient (post-transplant), and sample from stem cell product | End of treatment |
Countries
United States
Outcome results
None listed