DNA Analysis of Tissue From Patients With T-Cell Acute Lymphoblastic Leukemia

Molecular Mechanisms of NOTCH Induced Transformation in T-ALL

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT00899366

Enrollment

Registered

2009-05-12

Start date

2006-12-28

Completion date

2009-07-30

Last updated

2020-02-05

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Leukemia

Keywords

T-cell adult acute lymphoblastic leukemia, T-cell childhood acute lymphoblastic leukemia, untreated adult acute lymphoblastic leukemia, untreated childhood acute lymphoblastic leukemia, recurrent adult acute lymphoblastic leukemia, recurrent childhood acute lymphoblastic leukemia, adult acute lymphoblastic leukemia in remission, childhood acute lymphoblastic leukemia in remission

Brief summary

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This laboratory study is looking at tissue samples from patients with T-cell acute lymphoblastic leukemia.

Detailed description

OBJECTIVES: * Identify the transcriptional signatures associated with the presence of NOTCH1 mutations in primary T-cell acute lymphoblastic leukemia (T-ALL) cells using oligonucleotide microarrays. * Characterize the global changes on gene expression resulting from the inactivation of NOTCH signaling in human T-ALL lymphoblasts. OUTLINE: This is a multicenter study. Frozen lymphoblast samples from patients with T-cell acute lymphoblastic leukemia (NOTCH1-mutated and wild type) are assessed for genetic expression profiles and mutations by microarray analysis and activated NOTCH1 protein by western blot analysis. PROJECTED ACCRUAL: A total of 48 samples from patients will be accrued for this study.

Interventions

GENETICmicroarray analysis

GENETICmutation analysis

OTHERimmunologic technique

Study design

Observational model

OTHER

Time perspective

RETROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to 120 Years

Healthy volunteers

Inclusion criteria

DISEASE CHARACTERISTICS: * Confirmed diagnosis of T-cell acute lymphoblastic leukemia * Frozen lymphoblast samples available * NOTCH1-mutated or wild type sample available PATIENT CHARACTERISTICS: * Not specified PRIOR CONCURRENT THERAPY: * Not specified

Exclusion criteria

* Not applicable

Design outcomes

Primary

Measure	Time frame	Description
Transcriptional signatures associated with NOTCH1 mutations in T-cell acute lymphoblastic leukemia (T-ALL) cells	1 month	Mutational analysis
Global changes on gene expression resulting from inactivation of NOTCH signaling in T-ALL cells	1 month	Gene expression

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026