Colorectal Cancer, Healthy, no Evidence of Disease, Precancerous Condition
Conditions
Keywords
healthy, no evidence of disease, stage I colon cancer, stage II colon cancer, stage III colon cancer, stage IV colon cancer, stage I rectal cancer, stage II rectal cancer, stage III rectal cancer, stage IV rectal cancer, recurrent rectal cancer, recurrent colon cancer, adenomatous polyp
Brief summary
RATIONALE: Studying samples of blood and tissue in the laboratory from patients with cancer, patients with colorectal polyps and from patients without polyps may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research study is looking at biomarkers in blood and tissue samples from patients with colorectal cancer or colorectal polyps and from patients without polyps (healthy volunteers).
Detailed description
OBJECTIVES: Primary * Perform metabolomic, lipidomic, glycoproteomic, proteomic, and genomic (OMIC) profiling using blood and tissue samples from patients with colorectal cancer (CRC) or colorectal adenomatous polyps and from patients without polyps. * Create an OMIC profile to predict the risk of CRC based on differences observed between patients with CRC, patients with colorectal adenomatous polyps, and patients without polyps. Secondary * Create an OMIC profile to predict response and toxicity to specific chemotherapies, biological therapies, and radiotherapy for CRC. * Utilize a novel knowledge discovery tool (BioMap) based on literature mining and, in the future, utilize the results of the OMIC analyses to identify interactive molecular pathways that underlie the development and progression of CRC. OUTLINE: Patients with locally advanced or metastatic colorectal cancer are stratified according to treatment (first-line chemotherapy with fluorouracil \[5-FU\]/oxaliplatin or 5-FU/irinotecan vs second- or third-line chemotherapy with irinotecan only vs biological therapy with bevacizumab vs biological therapy with cetuximab vs radiotherapy). Blood and tissue samples are collected periodically for laboratory studies. Samples are analyzed for metabolomics by nuclear magnetic resonance, gas chromatography, liquid chromatography, and mass spectrometry; lipidomics, proteomics, and glycoproteomics by liquid chromatography and mass spectrometry; and genomics (single nucleotide polymorphism biomarkers) by PCR. Vitamin D status is also assessed. Patients complete diet-history and lifestyle questionnaires at baseline and once a year for 2 years. Healthy volunteers complete these questionnaires only at baseline. After completion of study, patients are followed every 3 months for 2 years, every 6 months for 3 years, and then annually thereafter. Healthy volunteers are not followed after study completion.
Interventions
GENETICgene expression analysis
gene expression analysis
GENETICpolymerase chain reaction
polymerase chain reaction
GENETICpolymorphism analysis
polymorphism analysis
GENETICprotein expression analysis
protein expression analysis
GENETICproteomic profiling
proteomic profiling
OTHERgas chromatography
gas chromatography
OTHERlaboratory biomarker analysis
laboratory biomarker analysis
liquid chromatography
OTHERmass spectrometry
mass spectrometry
OTHERquestionnaire administration
questionnaire administration
Sponsors
National Cancer Institute (NCI)
Indiana University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
DISEASE CHARACTERISTICS: * Meets 1 of the following criteria: * Diagnosis of one of the following: * Stage I or II colorectal cancer (CRC)\* * Planning to undergo surgery only * Stage III CRC\* * Planning to undergo surgery followed by adjuvant chemotherapy with or without neoadjuvant chemoradiotherapy * Stage IV CRC * Planning to undergo chemotherapy and biologic therapy (bevacizumab, cetuximab, or panitumumab) * Colorectal adenomatous polyps * Planning to undergo colonoscopy * Healthy volunteer * Planning to undergo colonoscopy NOTE: \*Patients with previously resected stage II or III CRC are eligible provided they undergo blood sample collection prior to starting chemotherapy PATIENT CHARACTERISTICS: * Not pregnant * Able to undergo an 8-hour overnight fast prior to metabolomic testing * Able to attend follow-up or treatment visits for up to 24 months for collection of blood samples * No prior or concurrent invasive cancer other than CRC (for patients with CRC) * No prior invasive cancer and no first-degree relative with a known history of CRC (for healthy volunteers and patients with colorectal polyps) PRIOR CONCURRENT THERAPY: * See Disease Characteristics
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Metabolomic, lipidomic, glycoproteomic, proteomic, and genomic (OMIC) profiling | End of Study |
| Creation of an OMIC profile to predict the risk of colorectal cancer (CRC) | End of Study |
Secondary
| Measure | Time frame |
|---|---|
| Creation of an OMIC profile to predict response and toxicity to specific therapies for CRC | End of Study |
| Identification of interactive molecular pathways that underlie the development and progression of CRC | End of Study |
Countries
United States
Outcome results
None listed