Lung Cancer
Conditions
Keywords
lung cancer
Brief summary
RATIONALE: Studying samples of tumor tissue and blood in the laboratory from patients with cancer may help doctors learn more about changes that occur in genetic material (DNA and RNA) and may also identify protein expression patterns related to cancer. It may also help doctors predict how patients will respond to treatment. PURPOSE: This research study evaluates changes in DNA, RNA, and proteins with the goal of predicting response to treatment in patients with lung cancer.
Detailed description
OBJECTIVES: * To determine protein and/or RNA expression patterns capable of predicting tumor response to therapy in tumor tissue samples from patients with lung cancer or suspected of having lung cancer. * To characterize the genes and proteins found to be predictive of response in order to help elucidate the molecular biology underlying cancer chemosensitivity. * To evaluate DNA mutations found within the lung cancer sample which may be predictive of response or resistance to certain therapeutic agents. OUTLINE: Patients undergo collection of tumor tissue by percutaneous fine needle aspiration, core biopsy, thoracentesis, or during any medically indicated procedure involving removal of lung cancer tissue. Tissue samples are analyzed by a variety of techniques, including DNA sequencing, RNA sequencing and expression levels, protein assessment \[by immunohistochemistry, western blot, Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-MS\]). The goal of these studies is to identify of gene mutations, gene expression levels, and proteins predictive of treatment response. Blood samples are also collected to obtain normal DNA for analysis. After completion of study, patients will be followed until their death.
Interventions
GENETICDNA/RNA sequencing and expression levels
Lung tumor tissue will be collected.
GENETICProtein expression analysis
Lung tumor tissue will be collected.
PROCEDURELung tumor biopsy
Tissue will be collected by percutaneous fine needle aspiration, a percutaneous core biopsy, or during a medically indicated procedure during which lung tumor tissue will be removed
PROCEDUREBlood sample
Patients will be asked for a venous blood sample
Sponsors
National Cancer Institute (NCI)
Vanderbilt-Ingram Cancer Center
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
No minimum to 120 Years
Healthy volunteers
No
Inclusion criteria
* Diagnosis of suspected lung cancer or lung cancer
Exclusion criteria
* Inability to undergo therapy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| DNA, RNA, and Protein expression patterns and mutational analysis | After lung tumor tissue and blood collection. | DNA, RNA, and Protein expression patterns and mutation status in lung tumor tissue that are capable of predicting tumor response to therapy |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Characterization of genes and proteins predictive of response to therapy | After lung tumor tissue and blood collection | Identify genes and proteins predictive of response to therapy and that can be used to identify novel mechanisms underlying lung cancer behavior |
Countries
United States
Outcome results
None listed