Recurrent Wilms Tumor and Other Childhood Kidney Tumors, Stage III Wilms Tumor, Stage II Wilms Tumor, Stage IV Wilms Tumor, Stage I Wilms Tumor, Stage V Wilms Tumor
Conditions
Brief summary
This laboratory study is using gene expression profiling to identify different categories of Wilms tumors. Studying the genes expressed in samples of tumor tissue from patients with cancer may help doctors identify biomarkers related to cancer.
Detailed description
OBJECTIVES: I. Identify new molecular categories of Wilms tumor (WT) based on the gene expression profiles of samples from patients with this disease. II. Develop a classifying system (classifier) that will predict a defined number of clinically relevant categories based on expression of an established set of genes. III. Confirm and test the classifier with further analysis of the genes by several different methodologies, using additional patient samples that are outside of the case/cohort but which possess the clinically or scientifically relevant parameter. IV. Validate the use of the classifier to predict defined groups within a second set of samples (for which investigators are blinded to the clinical and biological data) by subsequent comparison with true incidence of pertinent markers. OUTLINE: Three hundred tumor specimens are analyzed for genetic expression profiles using Affymetrix assays. Specific genes are identified as classifiers and analyzed using tissue arrays. An additional 300 specimens are examined for gene expression and categorized according to the classifiers.
Interventions
OTHERlaboratory biomarker analysis
Correlative studies
Sponsors
National Cancer Institute (NCI)
Children's Oncology Group
Study design
Observational model
COHORT
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* Specimens from patients previously enrolled as On Study or Followed Biology Only on protocol NWTSG-5 (COG-Q9401) * Study follow-up data for disease status available * National Wilms Tumor Study Group/Children's Oncology Group pathology review showing favorable histology * Sufficient biology samples available without using biology bank reserves
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| New molecular categories of Wilms tumor identified and measured by gene expression profiles | Up to 6 years | — |
| Identification of genes that as a whole provide strong prediction of outcomes of interest | Up to 6 years | Supervised methods such as CART or Shrunken Centroid Classifier will be used. |
Countries
United States
Outcome results
None listed