Overweight
Conditions
Keywords
Inflammation, Fatty acids, Omega 3, Leukotrienes, Adiponectin, Adipose tissue, RNA, Messenger
Brief summary
The aim of this study is to investigate the effects of acute and short-term intervention with fish oil on inflammatory markers in overweight subjects.
Detailed description
Being overweight is associated with metabolic syndrome, cardiovascular disease, and diabetes mellitus (type 2). Adipose tissue can be regarded as an active organ, which produces an array of inflammatory mediators. This creates a state of chronic low-grade inflammation. Inflammation has been proven to be a key element in the development of atherosclerotic plaques. The marine n-3 polyunsaturated fatty acids (PUFAs) have beneficial effects on cardiovascular disease. These effects are partly attributed to the anti-inflammatory effect of the n-3 PUFAs. Previous studies have shown an anti-inflammatory effect of n-3 PUFAs in overweight subjects, but the studies have been conducted with a high daily dose of n-3 PUFA. We want to examine the effect of a lower dose of 1 g n-3 PUFA/day (the dose recommended by the Danish Heart Foundation). The study is randomized, placebo-controlled and double blinded. Fifty subjects aged 30 - 75 years (postmenopausal women; waist circumference \> 80 cm and men; waist circumference \> 94 cm) will be included. Blood samples and fatty tissue biopsies will be collected at baseline, after 1 day and after 6 weeks.
Interventions
DIETARY_SUPPLEMENTfish oil
Capsules. Approximately 1000 mg n-3 PUFA daily. One day + 6 weeks
DIETARY_SUPPLEMENTolive oil
Capsules. Approximately 1 g olive oil daily. One day + 6 weeks
Sponsors
Aalborg University Hospital
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
30 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Waist circumference ≥ 80 cm (females) or ≥ 94 cm (males) * Postmenopausal (females)
Exclusion criteria
* Daily intake of fish oil capsules * Daily intake of NSAIDs * HbA1c ≥ 8% * Serum creatinine ≤ 30 mL/min * Chronic inflammatory disease * Other serious illness * Inability to informed consent
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| To assess the effect of 1 g/day of n-3 polyunsaturated fatty acids on inflammatory markers | After 1 day and after 6 weeks intervention |
Secondary
| Measure | Time frame |
|---|---|
| To assess effect of 1 g n-3 polyunsaturated fatty acids (daily dose) on the fatty acid profile of plasma, leucocytes and adipose tissue. | After 1 day and 6 after weeks of intervention |
| To assess the effect of 1g n-3 polyunsaturated fatty acids (daily dose) on the gene expression profile (mRNA) - especially genes involved in encoding the inflammatory response. | After 1 day and 6 after weeks of intervention |
Countries
Denmark
Outcome results
None listed