Healthy
Conditions
Brief summary
Hydrolysis of dietary protein may impact their gastrointestinal kinetics and further metabolism. The primary goal of this work was to measure the metabolic fate of intact or hydrolyzed protein using a protein of good nutritional quality, i.e., casein. A second aim of this study was to assess the true ileal digestibility of dietary protein using different methods including the hydrolyzed casein method and to measure its possible effect on endogenous intestinal N losses in humans.
Interventions
OTHERIntestinal and metabolic exploration
Subjects are studied for 8h following the ingestion of a standard meal containing 15% of energy as protein. Intestinal, blood and urine are collected at regular intervals.
DIETARY_SUPPLEMENT15N-labelled intact casein
standard mixed meal containing 15% of energy as 15N-labelled intact casein
DIETARY_SUPPLEMENT15N-labelled hydrolyzed casein
standard mixed meal containing 15% of energy as 15N-labelled hydrolyzed casein
DIETARY_SUPPLEMENTmixture of AA
standard mixed meal containing 15% of energy as a mixture of AA mimicking the composition of casein but devoid in serine
Sponsors
Riddet Institute
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy subjects * BMI between 18 and 25
Exclusion criteria
* allergy to dairy proteins * positive serology to AgHbS, HCV or HIV * pregnancy * abusive drug or alcohol consumption.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Postprandial metabolic fate of dietary N | 0h-1h-2h-3h-4h-5h-6h-7h-8h postprandial |
Secondary
| Measure | Time frame |
|---|---|
| Differences in ileal digestibility determination according to the method used | 8h postprandial |
Countries
France
Outcome results
None listed