HIV Infections, Staphylococcal Infections
Conditions
Keywords
HIV, Methicillin-resistant Staphylococcus aureus (MRSA), HIV and Staphylococcus aureus infection
Brief summary
The purpose of this study is to investigate the role of T helper 17 cells (Th17) in the pathogenesis of MRSA infections.
Interventions
None listed
Sponsors
Infectious Diseases Clinical Research Program
National Institute of Allergy and Infectious Diseases (NIAID)
Henry M. Jackson Foundation for the Advancement of Military Medicine
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
All participant inclusion criteria: * Greater or equal to 18 years of age * Willingness to undergo blood draw. Skin biopsy will be requested, but is optional -AND- HIV-positive and MRSA-negative Group: * Documented positive HIV test result * Negative colonization swabs for S. aureus within 14 days of enrollment * No evidence of skin/soft tissue infection HIV-positive and MRSA-Colonization Group: * Documented positive HIV test result * History of of colonization with MRSA w/in 14 days of study enrollment HIV-positive and MRSA Infection Group: * Documented positive HIV test result * Skin/soft tissue infection with a positive wound culture showing MRSA within 7 days of enrollment * MRSA infection is not associated with an intravenous catheter or other nosocomial procedure HIV Negative groups: * Same criteria used for the HIV-negative groups as listed above. * No history of HIV infection. * Willing to undergo an HIV blood test, which must have a negative result.
Exclusion criteria
* Women with positive urine pregnancy test within 7 days of study enrollment * Women who are within 6 months of being postpartum or who are currently breastfeeding * Subjects unable or unwilling to complete questionnaires and blood draw.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| To compare distribution of Th17 cells and their functionality, in the peripheral blood of HIV-positive patients who are infected with MRSA with that of HIV-positive patients who are not colonized or infected with Staphylococcus aureus. | 1 year |
Secondary
| Measure | Time frame |
|---|---|
| Compare distribution of Th17 cells in the peripheral blood of groups of HIV-positive and HIV-negative participants who are colonized with MRSA as well as those who have a MRSA infection. | 1 year |
| Examine distribution of T cells, B cells, macrophages, dendritic cells, neutrophils, defensins, and IL-17 in T cell subsets in the skin | 1 year |
| Compare Th17 cells in peripheral blood of HIV-negative participants with MRSA infection with that of HIV-negative subjects not colonized of infected with Staph aureus. | 1 year |
| Collect information on factors that may play a role in development of MRSA colonization/infection. Includes demographic, hygienic, exercise-related, and sexual factors which may contribute to MRSA. | 1 year |
Countries
United States
Outcome results
None listed