Allergy
Conditions
Brief summary
The aim of this study is to show that flow cytometry can be an accurate tool to help physicians regarding the diagnosis, the SIT decision and the SIT arrest of hymenoptera venom allergy. 75 patients having a story of reaction to hymenoptera venom will be selected for this trial. Blood samples will be analyzed at: inclusion visit, Week 1 visit, Week 3 visit, Week 10 and Week 21 visit.
Interventions
OTHERflow cytometry
flow cytometry
Sponsors
University Hospital, Limoges
Study design
Allocation
NON_RANDOMIZED
Intervention model
SINGLE_GROUP
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
No
Inclusion criteria
* Adult patient (18 - 70 years) * Patient being informed and accepting to participate in the study with signature of informed consent * Patients having a clinical systematic and local, loco-regional story of reaction to the piqures of hymenopterans. * Patients treated by SIT for at least 5 years * Patients benefiting from a national insurance health
Exclusion criteria
* Children * Pregnant woman or who breast-feed * Patient under antihistaminic or corticoid for less than 8 days before the blood drawing * Protected Patient * Patient not having given his agreement after it was informed
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| % CD63 expression on basophil membrane by flow Cytometry | Inclusion , Week 1, Week 3, Week 10 and Week 21 visits |
Secondary
| Measure | Time frame |
|---|---|
| Blood blocking factors level | Week 1, Week 3, Week 10 and Week 21 visits |
Countries
France
Outcome results
None listed