Infertility
Conditions
Keywords
Infertility, Assisted reproductive technology, Nitric oxide
Brief summary
Improvements in In-Vitro Fertilization (IVF) outcomes have largely been due to the generation of excess embryos allowing for multiple embryo transfer. Unfortunately this has also increased the risk of multiple pregnancy with associated maternal and neonatal morbidity and mortality. The investigators believe that the concentration of nitric oxide metabolites measured in the culture medium from the first day of culture can identify the best quality embryos allowing us to transfer a limited number of embryos without compromising the optimum chance for pregnancy.
Interventions
PROCEDURETransfer of embryos on day 3 or day 5 after fertilization.
Subjects will be allocated at random to the two arms. Group 1 will have two embryos transferred on day 3 based on nitric oxide concentration. Group 2 will have two embryos transferred on day 5 based on morphologic criteria.
PROCEDUREDay 5 transfer
Subjects will have embryo transfer on day 5 after fertilization. Selection of embryos to transfer will be based on morphologic criteria.
Sponsors
Johns Hopkins University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
21 Years to 37 Years
Healthy volunteers
Yes
Inclusion criteria
* Age 21-37 * Female * Less than two previous unsuccessful IVF cycles
Exclusion criteria
* Day 3 FSH \>9.9 * Fewer than 8 mature oocytes retrieved * Fewer than 8 normally fertilized embryos on day 2 after fertilization
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Percentage of subjects with viable pregnancy one month after embryo transfer | 1 month |
Secondary
| Measure | Time frame |
|---|---|
| Percentage of subjects with > 2 viable embryos one month after embryo transfer | 1 month |
Countries
United States
Outcome results
None listed