Type 2 Diabetes Mellitus
Conditions
Keywords
metabolomics, flow-mediated vasodilation
Brief summary
The purpose of this study is to identify and characterize specific chemicals and metabolic pathways that change with forearm ischemia. These changes will be compared with forearm blood flow and flow-mediated vasodilation of the brachial artery.
Interventions
PROCEDUREblood sampling and brachial artery ultrasound
Intravenous blood sampling will be performed before and 15 seconds, 30 seconds, 1,2,5,10,and 30 minutes following an ischemic stimulus (inflation of a blood pressure cuff to suprasystolic pressure for 5 minutes) Brachial artery ultrasound imaging will be performed before and 1 minute after a 5 minute ischemic stimulus, to assess endothelium-dependent vasodilation of the brachial artery. Following completion of blood sampling, an additional resting image of the brachial artery will be obtained. Sub-lingual nitroglycerin will be administered, and the artery will be imaged again after 3 minutes, to assess endothelium-independent vasodilation.
Sponsors
Brigham and Women's Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Adults age 18 or older * Type 2 diabetes mellitus
Exclusion criteria
* Type 1 diabetes mellitus * Type 2 diabetics taking insulin, thiazolidinediones (rosiglitazone or pioglitazone), an amylin analog (Symlin \[pramlintide\]), or incretin mimetics (Byetta \[exenatide\]) * Pregnancy * Uncontrolled hypertension, \> 180/100 mmHg
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| change from baseline (pre-forearm ischemia) in circulating metabolites compared with post-ischemic forearm measurements | pre/post 5 minute ischemic stimulus |
Countries
United States
Outcome results
None listed