Irritable Bowel Syndrome
Conditions
Keywords
serotonin, gut permeability, visceral sensitivity, irritable bowel syndrome
Brief summary
Serotonin, 5-hydroxytryptamin (5-HT), plays an important role in regulating the gastrointestinal functions. In this study we will modulate the serotonin system with acute tryptophan depletion (ATD) and administration of 5-hydroxytryptophan (5-HTP). ATD is based on ingestion of an amino acid drink devoid of the precursor of serotonin and hence caused a decreased serotonin level. 5-hydroxy-tryptophan is the direct precursor of serotonin and its administration, an increased serotonin level is expected. During these interventions, gut permeability and visceral sensitivity will be measured in healthy individuals and patients with irritable bowel syndrome, which is characterized by altered permeability and visceral hypersensitivity.
Interventions
DRUGOxitriptan
100 mg 5-Hydroxytryptophan will be administered orally.
Acute tryptophan depletion employs the oral ingestion of an amino acid mixture devoid of tryptophan, the precursor of serotonin, which results in lowered serotonin levels.
OTHERAmino acid drink with tryptophan
Amino acid drink with tryptophan, placebo for the ATD procedure
DRUGPlacebo capsule
Placebo for 5-HTP
Sponsors
Top Institute Food and Nutrition
Maastricht University Medical Center
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Caregiver, Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* IBS-patients Inclusion criteria: 1. IBS will be diagnosed according to the Rome III criteria\* \[35\]: Recurrent abdominal pain or discomfort at least 3 days per month in the last 3 months associated with 2 or more of the following: * Improvement with defecation * Onset associated with a change in frequency of stool * Onset associated with a change in form (appearance) of stool * Criteria fulfilled for the last 3 months with symptom onset at least 6 months prior to diagnosis * Discomfort means an uncomfortable sensation not described as pain. In pathophysiological research and clinical trails, a pain/discomfort frequency of at least 2 days a week during screening evaluation is an indication for subject's eligibility. 2. Based on the medical history and previous examination, no other causes for the abdominal complaints can be defined. 3. Age between 18 and 65 years Healthy individuals Inclusion criteria: All subjects will be screened with a standardized psychiatric examination using the mini international neuropsychiatric interview (MINI) to determine present psychiatric state. General psychological state will be assessed using the 17 item Hamilton depression rating scale (HAM-D17), the Dutch version of the symptom checklist (SCL-90) and the hospital anxiety and depression rating scale (HADS). The psychiatric evaluation will be carried out by a psychiatrist. Volunteers with deviating scores on any topic will be excluded from participation. Healthy individuals between age 18 and 65 years will be included in the study.
Exclusion criteria
*
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary aim is to assess intestinal permeability under altered serotonergic conditions by means of sugar permeability test | 2010 |
Secondary
| Measure | Time frame |
|---|---|
| To assess tight junction functionality and serotonin metabolism To assess visceral hypersensitivity under altered serotoninergic conditions To examine biopsy specimens for serotonergic and intestinal permeability parameters in ex vivo circumstances | 2010 |
Countries
Netherlands
Outcome results
None listed