Immune System
Conditions
Keywords
Probiotics, Immunity, Immune response, Intestinal microflora, Blood lipids, Phagocytosis activity
Brief summary
The purpose of this study is to determine the dose-response effect of a yogurt containing a combination of bifidobacterium Lactis (BB-12) and Lactobacillus Acidophilus (LA-5) and green tea extract on markers of the immune system and gut health in healthy subjects. We hypothesize that the response of the immune function will be dose-dependent of the probiotics found in the fermented yogurt.
Detailed description
There is an increasing list of food containing probiotics on the market. Several studies have emphasized the health benefits of single probiotics, particularly on the immune system. However, it is unclear how a combination of two different probiotics complemented with green tea extract can beneficially modify markers of the immune. The aim of this study is to determine the dose-response effect of a yogurt containing a combination of bifidobacterium lactis (BB-12) and Lactobacillus Acidophilus LA-5 and green tea extract on immune system in healthy subjects. More specifically, this randomized, parallel placebo controlled study will investigate the impact of increasing doses (109 and 1010) of these probiotics on phagocytosis activity, oxidative metabolism and on the antipneumococcics antibody specific serotype response S. pneumoniae vaccination. Finally, this study will also examine the effect of increasing dose of these probiotics on intestinal microflora and blood lipids.
Interventions
DIETARY_SUPPLEMENTYogurt with 1X10E9 BB-12 and LA-5
100g of yogurt containing the probiotics L.acidophilus and B.lactis (concentrations of 1x10E9 of each probiotics) with 40 mg of green tea extract, once a day for 10 weeks.
DIETARY_SUPPLEMENTYogurt with 1X10E10 BB-12 and LA-5
100g of yogurt containing the probiotics L.acidophilus and B.lactis (concentrations of 1x10E10 of each probiotics) with 40 mg of green tea extract, once a day for 10 weeks.
DIETARY_SUPPLEMENTYogurt Placebo
100g of yogurt placebo containing no probiotics and no green tea extract, once a day for 10 weeks.
Sponsors
Aliments ULTIMA Foods Inc.
McGill University
TransBiothec
Laval University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 55 Years
Healthy volunteers
Yes
Inclusion criteria
* Age between 18 to 55 years * Healthy individuals, non-smokers * BMI between 18 and 35 kg/m2 * Stable weight (+/- 5kg) for 3 months before randomisation * Agree to receive a vaccine Pneumovax 23®
Exclusion criteria
* Pregnant or lactating woman * Previous history of cardiovascular disease * Diabetes * Kidney or liver disease * Gastrointestinal disorders or diseases * Endocrine disorders or diseases * Allergy * Subjects taking hypolipidemic drugs, antidepressant, medication for high blood pressure, for inflammation or auto-immune diseases. * Subject who have receive antipneumococcics vaccine in the year before randomisation.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Phagocytosis activity and oxidative metabolism in phagocytes. | 6 weeks after vaccination |
Secondary
| Measure | Time frame |
|---|---|
| antipneumococcics antibody specific serotype response to S. pneumoniae vaccination. | 6 weeks after yogurt consumption and vaccination |
| Intestinal microflora characteristics. | 4 weeks after yogurt consumption |
| Influence of blood lipids. | After 4 weeks and 10 weeks of yogurt consumption |
Countries
Canada
Outcome results
None listed