Cystic Fibrosis
Conditions
Keywords
cystic fibrosis, folate, vitamin B12
Brief summary
The purpose of this study is to determine whether 5 - methyltetrahydrofolate and vitamin B12 supplementation can ameliorate cell plasma membrane features in pediatric patients with cystic fibrosis
Detailed description
Cystic fibrosis (CF) is one of the most common fatal autosomal recessive disorders in the Caucasian population caused by mutations of gene for the cystic fibrosis transmembrane conductance regulator (CFTR). New experimental therapeutic strategies for CF propose a diet supplementation to affect the plasma membrane fluidity and to modulate the cellular amplified inflammatory response in CF target organs. Red blood cells (RBCs) were used to investigate plasma membrane, because RBCs share lipid, protein composition and organization with other cell types.
Interventions
DIETARY_SUPPLEMENT5-methyltetrahydrofolate and vitamin B12
children with cystic fibrosis were supplementated for 6 months with 5-MTHF, 7,5 mg/day ; vitamin B12, 0,5 mg/day
Sponsors
Universita di Verona
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
3 Years to 8 Years
Healthy volunteers
Yes
Inclusion criteria
* cystic fibrosis
Exclusion criteria
* diabetes * chronic infections of the airways * regular antibiotics intake
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| We evaluated membrane protein oxidative damage of red cells | 6 months |
Secondary
| Measure | Time frame |
|---|---|
| we evaluated cation transport pathways of red cells | 6 months |
Countries
Italy
Outcome results
None listed