Dry Eye Disease
Conditions
Keywords
dry eye, Thiol redox, Oxidative stress
Brief summary
Dry eye disease is related with oxidative stress at the ocular surface. GSH is one of the key factor of protect from oxidative stress. To evaluate thiol status of ocular surface cells by monitoring GSH concentration, we can evaluate the severity of dry eye.
Detailed description
The conjunctival cells are collected by impression cytology. Those cells are stained with 100 mM of monochlorobimane (MCB, M-1381, Molecular Probes, Eugene, OR) and propidium iodide (PI). Fluorescent intensity, reflecting the amount of icGSH, was inspected under a confocal laser scan microscope. To detect the GSH levels we used a fluorescent MCB probe with excitation and emission wavelengths of 405 nm and 410-480 nm, respectively. The cell-permeating MCB probe is non-fluorescent but forms a fluorescent adduct with GSH in a reaction catalyzed by glutathione-S-transferase.
Interventions
None listed
Sponsors
Kyoto Prefectural University of Medicine
Meiji University of Oriental Medicine
Study design
Observational model
CASE_CONTROL
Eligibility
Sex/Gender
ALL
Age
20 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* dry eye patients * control volunteers
Exclusion criteria
* Young (less than 20) patients * The patients who rejected this study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Tear amount, tear break up time, and so on are measured clinically. The thiol redox status is measured by staining the membrane. | Once a month (totally 2-4 times) at the clinic |
Countries
Japan
Contacts
Primary ContactJun Yamada, MD, PhD.
Backup ContactNorihiko Yokoi, MD, PhD.
Outcome results
None listed