Cancer
Conditions
Keywords
bone marrow, antigen expression, volunteers
Brief summary
Antigen expression levels in bone marrow from volunteering donors will be analyzed. The results of this analysis will be used to establish a clinical test to identify cancer patients who could qualify to receive an investigational treatment being developed by GlaxoSmithKline.
Interventions
PROCEDUREBone marrow aspiration
Collection of a 2 ml bone marrow sample from an aspirate carried out for medical purposes.
Sponsors
GlaxoSmithKline
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* The donor is aged \>= 18 years * Written informed consent has been obtained prior to the performance of any specific procedure. * The donor has a medical indication for which a bone marrow aspiration is planned. * The donor is eligible for a bone marrow aspiration.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Number of copies of Wilms' Tumor 1 (WT1) antigen expression in bone marrow | At Day 0 | Establishment of the range of WT1 transcript levels in a collection of bone marrow samples by real-time quantitative RT-PCR (Reverse Transcription Polymerase Chain Reaction). Due to the low numbers, they were expressed as \[value x 10E-03\]. Only the samples with gene expression \>0 were presented. |
| Number of copies of PRAME (Preferentially expressed in melanoma)antigen expression in bone marrow | At Day 0 | Establishment of the range of PRAME transcript levels in a collection of bone marrow samples by real-time quantitative RT-PCR (Reverse Transcription Polymerase Chain Reaction). Due to the low numbers, they were expressed as \[value x 10E-03\]. Only the samples with gene expression \>0 were presented. |
Countries
Belgium
Outcome results
None listed