Use of TREM-1 Protein to Differentiate Viral and Bacterial Pneumonias in Intubated Children

Use of a Novel Protein (sTREM-1) to Differentiate Pure Viral Lung Infection From Viral With Co-existing Bacterial Lung Infection

Status

Withdrawn

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT00645619

Enrollment

Registered

2008-03-27

Start date

2008-03-24

Completion date

2008-03-26

Last updated

2018-12-26

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Bacterial Pneumonia, Viral Pneumonia

Keywords

Pneumonia,viral, Pneumonia,bacterial, TREM-1 protein,human

Brief summary

The purpose of this study is to determine whether a protein called TREM-1 can be used to differentiate viral and bacterial pneumonias in children who are on ventilator support. We propose that the level of TREM-1 will be significantly elevated in the lung fluid of children with bacterial pneumonia and viral with co-existing bacterial pneumonia than in children with pure viral pneumonia.

Detailed description

Most often, viruses are the cause of pneumonia in children. However, viral pneumonias are frequently associated with secondary bacterial pneumonia. It is important, though difficult, to differentiate patients who only have viral pneumonia from those who have viral pneumonia with secondary bacterial pneumonia. This will help physicians to prescribe antibiotics to only those with bacterial pneumonia and avoid antibiotic use in those with pure viral pneumonia, thus help to limit health-care cost and to decrease emergence of antibiotic resistance. In adult studies, TREM-1 has been shown to be specifically expressed in bacterial infections. We propose that measuring TREM-1 in the bronchoalveolar lavage (BAL) fluid will help to differentiate these groups. Our hypothesis is that concentration of TREM-1 will be significantly elevated in the BAL fluid of children with bacterial pneumonia and viral with co-existing bacterial pneumonia than in children with pure viral pneumonia.

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

CROSS_SECTIONAL

Eligibility

Sex/Gender

ALL

Age

No minimum to 18 Years

Healthy volunteers

Inclusion criteria

* Children from birth to 18 years intubated for respiratory failure or for surgery as mentioned above within 48 hours of intubation.

Exclusion criteria

* Use of antibiotics \>72 hours preceding the study (not applicable to the definite bacterial pneumonia group) * Use of oral/parenteral glucocorticoid therapy \<2 weeks prior to admission * Presence of tracheostomy * Active treatment for pulmonary arterial hypertension * Mechanical ventilation with FIO2 \>0.6, MAP\>20 * Presence of severe pulmonary interstitial emphysema, pneumothorax, bradycardia (heart rate, \<80 beats/min in neonates, \<70 beats/min in infants), hypotension (mean arterial pressure, \<40 mm Hg in neonates, \<50 mm Hg in infants), and platelet count of \<30,000/mm3. * Immunodeficient or immunocompromised due to other conditions. * Enrollment in another interventional study that employs BAL.

Design outcomes

Primary

Measure	Time frame
TREM-1 level in the BAL fluid of patients with pure viral pneumonia in comparison to patients with viral with co-existing bacterial pneumonia	Within 48 hours of being intubated

Secondary

Measure	Time frame
TREM-1 level in the BAL fluid of patients with pure bacterial pneumonia and no pneumonia	Within 48 hours of intubation for TREM-1 level
TREM-1 level in the serum of all 4 groups	Within 48 hours of intubation for TREM-1 level
Length of ventilator support, length of ICU and hospital stay	Within 48 hours of intubation for TREM-1 level

Countries

United States

Outcome results