Fertility
Conditions
Brief summary
The aim of this study is to examine whether incubation of human oocytes until pronuclear stage, in different culture media containing high and low concentrations of glucose affect fertilization differently, not only in standard IVF cycles, but also after intracytoplasmatic sperm injection.In addition, we wish to determine whether exposure of oocytes to different concentrations of glucose until pronuclear stage has further effect on embryo quality and development until the blastocyst stage.
Interventions
OTHERUniversal IVF Medium + ISM1 Medium
Sibling oocytes will be equally divided and incubated separately in different 4-well culture dishes containing either IVF medium or ISM1 Medium and while ICSI will be returned to the same culture dish following injection. Fertilization assessment will be performed approximately after 15-20 hours for standard IVF and 12-18 hours post ICSI performance. After assessment, fertilized oocytes from different culture media will be transferred separately into fresh culture dishes containing ISM1 medium only. The zygotes will be incubated until embryo transfer on day 2 or 3. Embryo quality will be assessed according to the morphology of blastomeres as well as it's developmental stage.
Sponsors
Hillel Yaffe Medical Center
Study design
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Patients undergoing IVF treatment in our clinic during study period
Exclusion criteria
* Females older than 40 years * Patients with less than 6 retrieved oocytes * Male patients older than 50 years old * Patients after testis biopsies
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Fertilization rate and embryo quality | 6 days |
Outcome results
None listed