Burn Injury, Major Trauma
Conditions
Keywords
fibrinogen, burns, trauma, metabolism
Brief summary
To study the fibrinogen metabolic changes in relation to coagulation disorder in patients with severe burns and trauma injuries. As a result of the burn or trauma injury breakdown of fibrinogen is accelerated.
Detailed description
In this protocol, we will investigate fibrinogen metabolism in burn and trauma patients, using an 8h infusion of stable isotopes labeled amino acids (1-13C-phenylalanine, 2H5-phenylalanine, and 2H3-ketoisocaporate). The increase of the labeled amino acids incorporating into fibrinogen during the infusion and the decay of labeled fibrinogen after stopping the isotopes infusion (as measured by GC-MS) will be used to calculate fibrinogen synthesis and degradation. Stable isotopes are non-radioactive.
Interventions
None listed
Sponsors
United States Army Institute of Surgical Research
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* 20% total body surface area burn or more * severe trauma with injury severity score greater than 10 * between ages of 18 and 65 years
Exclusion criteria
* history of blood clotting disorder * history of liver disease such as cirrhosis or hepatitis * pregnant or nursing * allergic to iodine, shellfish or dye used in X-Rays * taking long term medications for blood clotting usch as Coumadin or Plavix * prisoners * receiving total parenteral nutrition
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| During the flow phase following burn or trauma injury, fibrinogen degradation is accelerated | 7 |
Secondary
| Measure | Time frame |
|---|---|
| Fibrinogen degradation stimulates fibrinogen synthesis at the expense of whole body protein catabolism | 7 days |
Outcome results
None listed