PCR-Based Detection of Bacterial Agents in Platelet Concentrates and Whole Blood

Healthy

bacterial contamination, platelet concentrates, blood donation, looxster, pureprove, bacterial genomic DNA, bacterial contamination of platelet concentrates, Preparation of bacterial DNA in blood, comparison of blood culture versus NAT

Using methods of molecular biology bacteria can be detected in blood. In comparison to conventional blood cultures bacterial DNA is isolated using a new sample preparation system in platelet apheresis concentrates and in the predonation whole blood of donors.

Bacterial contamination of platelet concentrates is discussed worldwide and according to literature occuring in 1:1000 to 1:5000 concentrates. Bacterial contaminated concentrates can in fact cause severe infections in patients. therefore this study initially intends to detect contamination with bacterial DNA in platelet aphereses concentrates. All sample donations are also examined by blood culture for contamination. To exclude already existing infection of the donor one tube of whole blood is taken in addition to the other legally required samples for the production of pharmaceutical medicine. All samples are anonymised before passed on for analysing.

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Germany