Obstructive Sleep Apnea Syndrome
Conditions
Keywords
Pediatric Obstructive sleep apnea syndrome
Brief summary
The purpose of this study is to compare the cytokine profile of tonsillar and peripheral blood mononuclear cells in patients with obstructive sleep apnea (OSA) and to compare the blood mononuclear cell cytokine secretion profile between patients with obstructive sleep apnea and controls without apnea.
Detailed description
We will recruit subjects with OSA about to undergo adenotonsillectomy and collect a blood sample and portion of their tonsils after the induction of general anesthesia. The mononuclear cells will be isolated from both these tissues and stimulated with different markers and cytokines assayed and compared. We will also recruit patients with no symptoms of OSA undergoing unrelated elective surgery and ascertain the absence of OSA by having the parents fill out a sleep questionnaire. We will then collect blood samples from these patients after the induction of anesthesia and examine the cytokine secretion profile and compare it to that of the children with OSA.
Interventions
PROCEDUREtonsillectomy
medically indicated tonsillectomy in subjects with and without OAS
Sponsors
University of Chicago
Study design
Allocation
NA
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Months to 12 Years
Healthy volunteers
Yes
Inclusion criteria
* OSA documented by polysomnography and clinical symptoms. * Patients with no symptoms of OSA and negative sleep questionnaire.
Exclusion criteria
* Significant systemic disease except mild asthma
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| levels of cytokines released from PBMCs of OAS subjects and controls | just prior to surgery |
Secondary
| Measure | Time frame |
|---|---|
| cytokine level comparison between PBMCs and tonsillar lymphocytes within OAS groups | at time of surgery |
Countries
United States
Outcome results
None listed