Effect of Ischemic Postconditioning on Targeting of Annexin A5 After Forearm Exercise

Status

Withdrawn

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT00441714

Enrollment

Registered

2007-03-01

Start date

2007-10-31

Completion date

2007-12-31

Last updated

2015-11-10

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Cardiovascular Disease, Ischemia-reperfusion Injury, Postconditioning

Keywords

cardiovascular disease, ischemia-reperfusion injury, postconditioning, annexin targeting

Brief summary

To adjust our forearm model for ischemia reperfusion injury, in order to used it for assessment of ischemia reperfusion damage in elderly and patients. To test the effect of ischemic postconditioning on ischemia reperfusion injury in healthy volunteers, using Annexin binding after repetitive handgripping.

Detailed description

Recently, ischemic postconditioning has been identified as a protective intervention against ischemia-reperfusion injury. In animal studies, the signalling pathway of this (impressive) protective phenomenon is very similar to ischemic preconditioning. It opens a new avenue of post-reperfusion interventions with drugs that have been shown to mimic ischemic preconditioning. Before we can study this phenomenon in our forearm ischemia-reperfusion model, we need additional validating experiments. The purpose of this study proposal is to provide these data.

Interventions

PROCEDURErepetitive short ischemia and reperfusion of non dominant fore arm after 10 minute ischemic exercise

Study design

Allocation

RANDOMIZED

Intervention model

CROSSOVER

Primary purpose

DIAGNOSTIC

Masking

NONE

Eligibility

Sex/Gender

MALE

Age

18 Years to 50 Years

Healthy volunteers

Yes

Inclusion criteria

* Age 18-50 years * No physical limitation to perform ischemic exercise * Informed consent

Exclusion criteria

* Diabetes (fasting glucose \> 7,0mmol/l, or random glucose \>11,0mmol/l) * hyperlipidemia (random total cholesterol \> 6.5 mmol/l) * Hypertension (supine SBP/DBP \> 140/90 mmHg at screening) * Any cardiovascular disease * Drug abuse * Concomitant chronic use of medication * Administration of radioactivity in research setting during the last 5 years * Participation to any drug-investigation during the previous month as checked with VIP check according to CRCN standard procedures.

Design outcomes

Primary

Measure	Time frame
Annexin A 5 targeting in the non dominant thenar muscle after (ischemic exercise), as a indicator for ischemia reperfusion injury.	—

Secondary

Measure	Time frame
Workload during (ischemic) exercise.	—

Countries

Netherlands

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026