Obesity, Insulin Resistance
Conditions
Keywords
Obesity, Insulin resistance, Hypocaloric diet, Adipose tissue, adipocytokine
Brief summary
The aim of the study was to investigate the relationship between insulin resistance and body composition in relation to secterion and expression of adipocytokines. Obese subjects undergo 6 months hypocaloric diet and, before, during and at the end of the diet, plasma samples and subcutaneous adipose tissue samples are obtained for subsequent analysis. In addition, body composition and insulin resistance are measured before and during the diet.
Detailed description
The aim of the study was to investigate the relationship between insulin resistance and body composition in relation to secterion and expression of adipocytokines. Obese subjects undergo 6 months hypocaloric diet without any medication and, before, during and at the end of the diet, plasma samples and subcutaneous adipose tissue samples are obtained for subsequent analysis. Adipose tissue samples are obtained using needle biopsy. In addition, before and during the diet,body composition is measured using multifrequency bioimpedance and insulin resistance is evaluated using either hyperinsulinemic clamp or glucose/insulin - derived indices.
Interventions
BEHAVIORALHypocaloric diet
Sponsors
Charles University, Czech Republic
Study design
Allocation
NON_RANDOMIZED
Intervention model
SINGLE_GROUP
Primary purpose
PREVENTION
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 60 Years
Healthy volunteers
No
Inclusion criteria
* Obesity
Exclusion criteria
* Diabetes * Coronary heart disease * Eating disorders * Medication of beta blockers * Medication of antiobesity drugs
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Body fat | — |
| Body weight | — |
Secondary
| Measure | Time frame |
|---|---|
| Insulin resistance | — |
| Adipocytokine plasma levels | — |
| Adipocytokine adipose tissue expression | — |
Countries
Czechia
Outcome results
None listed