Diet, Obesity and Genes (DiOGenes)

Conditions

Cardiovascular Disease, Metabolic Syndrome, Obesity, Type 2 Diabetes

Keywords

Diogenes, Diet Intervention, Family, Glycemic Index, Protein

Brief summary

Study Title: Diogenes, A randomised controlled trial comparing the effect of reduced-fat diets varying in glycaemic index (high vs. low) and protein content (high vs. normal) on bodyweight in overweight and obese subjects after an initial weight loss.

Detailed description

DiOGenes is a randomised controlled dietary intervention study in obese/overweight families (adults and children) in 8 different European countries, testing the efficacy and safety of diets differing in Glycaemic Index (GI) and protein content. The 8 centres are divided into shop centers and instruction centers, that provide different kind of dietary intervention and for a different length(see below): The 2 Shop Centers (Denmark & The Netherlands): Dietary periods and clinical examinations: 1. Clinical examination 1 with anthropometry, blood etc. 2. 8 week Low Calorie Diet (LCD) period (800-1000 kcal/day)(Adults only) 3. Clinical examination 2 with anthropometry, blood etc. 4. 6 months dietary intervention period 1 (SUPERMARKET period)(Family) 5. Clinical examination 3 with anthropometry, blood etc. 6. 6 months dietary intervention period 2 (DIETARY INSTRUCTION period)(Family). 7. Clinical examination 4 with anthropometry, blood ect. 8. 12 months free-living period with no dietary intervention. (Family) 9. Follow up (only body weight registration) The 6 Instruction Centers (UK, Crete, Germany, Spain, Bulgaria, Czech Republic) Dietary periods and clinical examinations: 1. Clinical examination 1 with anthropometry, blood etc. 2. 8 week Low Calorie Diet (LCD) period (800-1000 kcal/day). (Adults only) 3. Clinical examination 2 with anthropometry, blood etc. 4. 6 months dietary intervention period (DIETARY INSTRUCTION period). (Family) 5. Clinical examination 3 with anthropometry, blood etc. 6. 6 months free-living period with no dietary intervention. (Family) 7. Follow up (only body weight registration) Note that, during the first 6 month intervention all families from the shop centers are provided all foods free of charge SUPERMARKET PERIOD, whereas in the Instruction Centers, the families receive dietary instruction only Instruction Only.

Kemper EJ, Blaak EE, Adriaens ME, Meex RCR, Meex RCR.

2025-10-10

10.1210/clinem/dgaf562

Identification of circulating apolipoprotein M as a new determinant of insulin sensitivity and relationship with adiponectin

Laurie Frances, Mikaël Croyal, Jean‐Bernard Ruidavets, Marie Maraninchi, Guillaume Combes et al. (14 authors)

International Journal of Obesity2024-03-155 citations

10.1038/s41366-024-01510-w

Dietary protein and the glycemic index handle insulin resistance within a nutritional program for avoiding weight regain after energy-restricted induced weight loss.

Vidal-Ostos F, Ramos-Lopez O, Jebb SA, Papadaki A, Pfeiffer AFH, Handjieva-Darlenska T, Kunešová M, Blaak EE, Astrup A, Martinez JA, Diet, Obesity, and Genes (Diogenes) Project.

2022-10-19

10.1186/s12986-022-00707-y

A fully joint Bayesian quantitative trait locus mapping of human protein abundance in plasma

Hélène Ruffieux, Jérôme Carayol, Radu Popescu, Mary‐Ellen Harper, Robert Dent et al. (10 authors)

PLoS Computational Biology2020-06-0325 citations

10.1371/journal.pcbi.1007882

Associations between the proportion of fat-free mass loss during weight loss, changes in appetite, and subsequent weight change: results from a randomized 2-stage dietary intervention trial.

Turicchi J, O'Driscoll R, Finlayson G, Duarte C, Hopkins M, Martins N, Michalowska J, Larsen TM, van Baak MA, Astrup A, Stubbs RJ.

2020-03-0140 citations

10.1093/ajcn/nqz331

Subcutaneous Adipose Tissue and Systemic Inflammation Are Associated With Peripheral but Not Hepatic Insulin Resistance in Humans

Birgitta W. van der Kolk, Marianthi Kalafati, Michiel Adriaens, Marleen M. J. van Greevenbroek, Nicole Vogelzangs et al. (18 authors)

Diabetes2019-09-0671 citations

10.2337/db19-0560

Salivary α-amylase copy number is not associated with weight trajectories and glycemic improvements following clinical weight loss: results from a 2-phase dietary intervention study

Armand Valsesia, Sameer S. Kulkarni, Julien Marquis, Patricia Leone, Polina Mironova et al. (13 authors)

American Journal of Clinical Nutrition2018-12-0714 citations

10.1093/ajcn/nqy363

Is abdominal obesity at baseline influencing weight changes in observational studies and during weight loss interventions?

Svendstrup M, Allin KH, Ängquist L, Schnohr P, Jensen GB, Linneberg A, Thuesen B, Astrup A, Saris WHM, Vestergaard H, Sørensen TIA.

2018-11-01

10.1093/ajcn/nqy187

Plasma metabolites and lipids predict insulin sensitivity improvement in obese, nondiabetic individuals after a 2-phase dietary intervention.

Meyer A, Montastier E, Hager J, Saris WHM, Astrup A, Viguerie N, Valsesia A.

2018-07-0120 citations

10.1093/ajcn/nqy087

Transcriptome profiling from adipose tissue during a low-calorie diet reveals predictors of weight and glycemic outcomes in obese, nondiabetic subjects.

Armenise C, Lefebvre G, Carayol J, Bonnel S, Bolton J, Di Cara A, Gheldof N, Descombes P, Langin D, Saris WH, Astrup A, Hager J, Viguerie N, Valsesia A.

2017-08-0958 citations

10.3945/ajcn.117.156216

Pretreatment fasting plasma glucose and insulin modify dietary weight loss success: results from 3 randomized clinical trials.

Hjorth MF, Ritz C, Blaak EE, Saris WH, Langin D, Poulsen SK, Larsen TM, Sørensen TI, Zohar Y, Astrup A.

2017-07-0574 citations

10.3945/ajcn.117.155200

Distinct lipid profiles predict improved glycemic control in obese, nondiabetic patients after a low-caloric diet intervention: the Diet, Obesity and Genes randomized trial.

Valsesia A, Saris WH, Astrup A, Hager J, Masoodi M.

2016-08-1029 citations

10.3945/ajcn.116.137646

The Impact of Gender and Protein Intake on the Success of Weight Maintenance and Associated Cardiovascular Risk Benefits, Independent of the Mode of Food Provision: The DiOGenes Randomized Trial.

Navas-Carretero S, Holst C, Saris WH, van Baak MA, Jebb SA, Kafatos A, Papadaki A, Pfeiffer AF, Handjieva-Darlenska T, Hlavaty P, Stender S, Larsen TM, Astrup A, Martinez JA.

2015-03-316 citations

10.1080/07315724.2014.948642

Adipose Tissue CIDEA Is Associated, Independently of Weight Variation, to Change in Insulin Resistance during a Longitudinal Weight Control Dietary Program in Obese Individuals

Émilie Montastier, Sébastien Dejean, Caroline Le Gall, Wim H. M. Saris, Dominique Langin et al. (6 authors)

PLoS ONE2014-07-0114 citations

10.1371/journal.pone.0098707

Analyses of single nucleotide polymorphisms in selected nutrient-sensitive genes in weight-regain prevention: the DIOGENES study.

Larsen LH, Angquist L, Vimaleswaran KS, Hager J, Viguerie N, Loos RJ, Handjieva-Darlenska T, Jebb SA, Kunesova M, Larsen TM, Martinez JA, Papadaki A, Pfeiffer AF, van Baak MA, Sørensen TIa, Holst C, Langin D, Astrup A, Saris WH.

2012-04-0430 citations

10.3945/ajcn.111.016543

Effects of weight loss and long-term weight maintenance with diets varying in protein and glycemic index on cardiovascular risk factors: the diet, obesity, and genes (DiOGenes) study: a randomized, controlled trial.

Gögebakan O, Kohl A, Osterhoff MA, van Baak MA, Jebb SA, Papadaki A, Martinez JA, Handjieva-Darlenska T, Hlavaty P, Weickert MO, Holst C, Saris WH, Astrup A, Pfeiffer AF, DiOGenes.

2011-11-21129 citations

10.1161/circulationaha.111.033274

A distinct adipose tissue gene expression response to caloric restriction predicts 6-mo weight maintenance in obese subjects.

Mutch DM, Pers TH, Temanni MR, Pelloux V, Marquez-Quiñones A, Holst C, Martinez JA, Babalis D, van Baak MA, Handjieva-Darlenska T, Walker CG, Astrup A, Saris WH, Langin D, Viguerie N, Zucker JD, Clément K, DiOGenes Project.

2011-10-2647 citations

10.3945/ajcn.110.006858

Blood profile of proteins and steroid hormones predicts weight change after weight loss with interactions of dietary protein level and glycemic index.

Wang P, Holst C, Andersen MR, Astrup A, Bouwman FG, van Otterdijk S, Wodzig WK, van Baak MA, Larsen TM, Jebb SA, Kafatos A, Pfeiffer AF, Martinez JA, Handjieva-Darlenska T, Kunesova M, Saris WH, Mariman EC.

2011-02-1427 citations

10.1371/journal.pone.0016773

Diets with high or low protein content and glycemic index for weight-loss maintenance.

Larsen TM, Dalskov SM, van Baak M, Jebb SA, Papadaki A, Pfeiffer AF, Martinez JA, Handjieva-Darlenska T, Kunešová M, Pihlsgård M, Stender S, Holst C, Saris WH, Astrup A, Diet, Obesity, and Genes (Diogenes) Project.

2010-11-01542 citations

10.1056/nejmoa1007137

Adipose tissue transcriptome reflects variations between subjects with continued weight loss and subjects regaining weight 6 mo after caloric restriction independent of energy intake.

Márquez-Quiñones A, Mutch DM, Debard C, Wang P, Combes M, Roussel B, Holst C, Martinez JA, Handjieva-Darlenska T, Kalouskova P, Jebb S, Babalis D, Pfeiffer AF, Larsen TM, Astrup A, Saris WH, Mariman E, Clément K, Vidal H, Langin D, Viguerie N, DiOGenes Project.

2010-08-2548 citations

10.3945/ajcn.2010.29808

Interventions

BEHAVIORALHigh or low Glycemic Index and high or low Protein Diet

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

PREVENTION

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

5 Years to 65 Years

Healthy volunteers

Yes

Inclusion criteria

* Families with healthy adults and children

Exclusion criteria

* Various

Design outcomes

Primary

Measure	Time frame	Description
For adults: body weight loss maintenance	6 Month	The primary outcome is related to body weight loss maintenance after 6 month of intensive, homogeneous and therefore comparable dietary intervention across all 8 centres.

Secondary

Measure	Time frame	Description
For adults: Drop out rate	6 month	Percentage drop out from randomisation to end of dietary intervention
For Adults: Change in risk factors for cardiovascular disease	6 month	Change in risk factors for cardiovascular disease
For children: Change in body weight	6 month	Change in body weight from randomization to end of intervention
For Children: Change in BMI-Z-score	6 month	Change in BMI-Z-score from randomisation to end of intervention
For adults: change in body composition	6 Month	The relative loss or gain of fat mass and fat free mass
For adults: More than 5% weight loss maintenance	6 month	Achievement of 5% of body weight loss from randomization
For adults: More than 10% weight loss maintenance	6 month	Achievement of 10% of body weight loss from randomization
For Adults: Change in risk factors for type-2 diabetes	6 month	Change in risk factors for type-2 diabetes

Other

Measure	Time frame	Description
For adults: Identify traits gene - diet interactions that determine responses to the dietary intervention.	12 months	To identify gene - diet interactions (e.g. SNP x dietary intake or copy number x dietary intake) that determine the family's and individual's responses to the dietary interventions.
For adults: Identify psychological traits that determine responses to the dietary intervention.	12 months	To identify psychological traits that determine the family's and individual's responses to the dietary interventions.
For adults: Identify traits biological that determine responses to the dietary intervention.	12 months	To identify biological traits that determine the family's and individual's responses to the dietary interventions.

Countries

Denmark

Outcome results

None listed