Type 2 Diabetes
Conditions
Keywords
Type 2 diabetes, physical training, mitochondrial function
Brief summary
Physical activity as treatment for patients with type 2 diabetes (T2D). Treatment of T2D is often done by medication, changing the diet and increased physical activity. It is well established that physical training has a positive effect on the pathology of T2D such as increased insulin sensitivity and reduced fasting plasma glucose (7) and blood lipids (15). It is also well known that in healthy subjects endurance training increases mitochondrial density, lipid oxidation during submaximal exercise, and results in a number of qualitative changes in the control of OXPHOS (24). However, the effect of physical training on the mitochondrial function in skeletal muscle of patients with T2D has not been investigated. The purpose of this project is to investigate the effect of physical training on the mitochondrial respiratory function in muscles of patients with T2D. Skeletal muscle biopsies will be taken before and after 10 weeks of physical training in patients with T2D and matched control subjects. Mitochondria will be isolated from the muscle biopsies, and respiratory function, free radical production and UCP3 will be determined. Furthermore, measures of maximal oxygen consumption (VO2max), heart rate and capillary lactate concentrations and carbohydrate and lipid oxidation will be determined partly to verify an increased aerobic capacity and partly to investigate the changes in these factors in T2D patients.
Interventions
PROCEDUREPhysical training (aerobic training)
Sponsors
Odense University Hospital
Study design
Allocation
RANDOMIZED
Intervention model
SINGLE_GROUP
Primary purpose
TREATMENT
Masking
SINGLE
Eligibility
Sex/Gender
MALE
Age
40 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Male, * BMI above 26, * Age between 40 and 65, * Living a sedentary lifestyle
Exclusion criteria
* Women * BMI under 26 * Age under 40 and over 65 * Trained subjects Control subjects: relatives to type 2 diabetic patients, T2D trial subjects
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Mitochondrial respiratory measurements. The investigations are carried out by two rounds - one before Christmas and one after. | — |
Secondary
| Measure | Time frame |
|---|---|
| Lipid oxidation | — |
| muscle fiber type composition | — |
| VO2max | — |
| mitochondrial-specific proteins (UCP3) | — |
| mitochondrial production of free oxygen radicals | — |
| muscle oxidative enzymes | — |
Countries
Denmark
Outcome results
None listed