Pilot Study on Shear-induced Platelet Aggregation in Acute Coronary Syndromes

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT00299143

Acronym

REACS

Enrollment

Registered

2006-03-06

Start date

2005-06-01

Completion date

2010-06-01

Last updated

2026-07-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Angina, Unstable

Keywords

SIPA, acute coronary syndrome

Brief summary

The purpose of this study is to determine whether shear-induced platelet aggregation is able to discriminate first acute coronary syndrome (ACS) from recurrent ACS

Detailed description

Predictive factors of recurrence of ACS are not well determined. Platelet aggregation and leucocyte activation seem to be involved in the pathogenesis. The aim of our study is to compare SIPA, platelet activation and platelet-leucocytes aggregates on the onset of the ACS and 3 months later in 2 groups of patients scheduled for a first episode of ACS or recurrent ACS .

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to 85 Years

Healthy volunteers

Inclusion criteria

over 18 years old. informed consent signed. acute coronary syndrome not associated with co-morbidity as bleeding diathesis or myocardial infarction during the procedure. clinical symptoms in agreement with myocardial infarction during the preceding 24 hours. CK OR troponin elevation and one or more of the following criteria: ECG modifications transient ST elevation\>1 mm. new inversion of ST \<1 mm on 2 contiguous derivations.

Exclusion criteria

Acute coronary syndrome with persistent ST elevation. Angioplasty in emergency before blood sampling. Inflammatory disease or cancer. Coagulation abnormalities. Antiphospholipid syndrome. Treatment by vitamin K antagonist. Severe disease with life expectancy lower than 2 years. One-year follow up impossible.

Design outcomes

Primary

Measure	Time frame	Description
Measurement of platelet aggregation induced by shear rates	Day 1, Month 3	Using rotational viscometer

Secondary

Measure	Time frame	Description
Measurement of platelet aggregation induced by ADP (Adenosine 5'-diphosphate)	Day 1, Month 3	Measured by turbidimetry in the of the agonist, at 37°C in an aggregometer (model 490, Chonolog)
Measurement of P-selectin membrane density	Day 1, Month 3	By flow cytometry (EPICS Profile, Beckman, Coulter)
Measurement of activated alphaIlbbeta3 membrane density	Day 1, Month 3	By flow cytometry (EPICS Profile, Beckman, Coulter)
Determination of the number of platelet-leucocyte aggregates in the circulating blood	Day 1, Month 3	By flow cytometry, using antibodies specific to platelets (CD41), polymorphonuclear cells (CD15) and monocytes labelled with a fluorochrome (FITC)
Measuring Von Willebrand factor	Day 1, Month 3	Carried out on frozen plasma of all patients

Countries

France

Contacts

STUDY_DIRECTORPhilippe G Steg, Professor

APHP

PRINCIPAL_INVESTIGATORNadine Ajzenberg, Dr

APHP, INSERM

Outcome results