PET Imaging of Brain 5-HT(1A) Receptors Using [(11)C](-)-RWAY

Healthy Volunteers

Serotonin, Positron Emission Tomograhy, Brain, Neurochemistry, Neurotransmitter, Healthy Volunteer, HV

This study will use positron emission tomography (PET) and magnetic resonance imaging (MRI) to measure a serotonin receptor subtype in the brain called 5-HT(1A). This receptor is a target for drug therapy to treat anxiety and depression. The study will see if a newly developed radioligand (radioactive substance used in PET scanning to study the receptor systems of the brain) called \[(11)C\](-)-RWAY is more effective than other radioligands currently used in brain receptor research. Healthy subjects 18-40 years of age may be eligible for this study. Candidates are screened with a physical examination, electrocardiogram, and blood and urine tests. Participants undergo PET and MRI scanning as follows: PET scan PET uses small amounts of a radioactive chemical called a tracer (in this case, \[(11)C\](-)-RWAY) that labels active areas of the brain. For the procedure, the subject lies on the scanner bed. A special mask is fitted to the subject's head and attached to the bed to help keep the head still during the scan so the images will be clear. A brief scan is done just before the tracer is injected to provide measures of the brain that will help in the precise calculation of information from subsequent scans. Then, the tracer is injected through a catheter (plastic tube) placed in the arm and pictures are taken for about 2 hours, while the subject lies still on the scanner bed. Subjects return to the clinic for blood and urine tests 24 hours after the scan. MRI The MRI scan is done within 1 year of the PET scan. MRI uses a magnetic field and radio waves to produce images of body tissues and organs-in this case, the brain. The subject lies on a table that is moved into the scanner (a tube-like device), wearing earplugs to muffle the noise of the machine during the scanning process. The space in the scanner is confining and may cause some people to be somewhat anxious. An intercom system allows the subject to speak with the staff member performing the study at all times during the procedure, and the procedure can be stopped at any time.

The 5-hydroxytryptamine (serotonin, 5-HT) 5-HT(1A) receptors subtype is a target for drug therapy in the treatment of anxiety and depression. Radioligands currently in use with PET for studying human brain 5-HT(1A) receptors in clinical research or drug development are based on WAY-100635. Though variously effective, they each suffer from one or more drawbacks with respect to measuring relative regional receptor densities. These drawbacks include: rapid metabolism and very low non-specific binding for \[carbonyl-(11)C\]WAY-100635; defluoridation for \[18F\]trans-FCWAY. Therefore, we have recently developed \[(11)C\](-)-RWAY as an alternative radioligand for brain 5 HT(1A) receptors. Even though similar to WAY-100635, \[(11)C\](-)-RWAY has a reversed amide linkage. Reversal of the direction of the amide bond may confer resistance to amide hydrolysis and make acceptable easy labeling at its O-methoxy group. In the present protocol, we plan to perform a kinetic brain imaging study in healthy human subjects to measure 5 HT(1A) receptors in brain regions with \[(11)C\](-)-RWAY.

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