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Drug Transport in Patients With Anorexia Nervosa

Influence of Cholesterol Plasma Levels on Drug Transport in Patients With Anorexia Nervosa

Status
UNKNOWN
Phases
Unknown
Study type
Observational
Source
ClinicalTrials.gov
Registry ID
NCT00176150
Enrollment
Unknown
Registered
2005-09-15
Start date
Unknown
Completion date
Unknown
Last updated
2005-09-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Anorexia Nervosa

Keywords

anorexia nervosa, multidrug resistance, cholesterol metabolism

Brief summary

The aim of this study is to investigate whether patients with anorexia nervosa have elevated plasma cholesterol levels and, if elevated plasma cholesterol levels influence drug transport by the ABC-transporter P-gp (P-glycoprotein, MDR 1/ABCB1). We will isolate peripheral blood mononuclear cells (PBMCs) and total RNA from the blood of patients with anorexia nervosa and healthy subjects as control. PBMCs will be used for quantitative cholesterol determination and for measuring the activity of P-gp using a FACS (fluorescence-activated cell sorting) method (rhodamine123 efflux). Total RNA will be used for quantitative expression analysis of P-gp by reverse transcriptase real time PCR.

Interventions

None listed

Sponsors

Heidelberg University
Lead SponsorOTHER

Study design

Observational model
DEFINED_POPULATION
Time perspective
OTHER

Eligibility

Sex/Gender
FEMALE
Age
15 Years to 30 Years

Inclusion criteria

Diagnosis anorexia nervosa according to DSM-IV (Diagnostic and Statistical Manual of Mental Disorders. Washington, DC) Refusal to gain weight to or over a minimal normal weight (BMI \< 17,5 kg/m²) Fear of gaining weight despite underweight

Exclusion criteria

Contraindication for taking blood samples (haemoglobin \< 8,5 g/dl) Medication which could influence the lipid metabolism (current and the last two months) Acute or chronic diseases, particularly malign diseases Diseases which could affect the safety of the patients or which could interfere with aims of the study Positive pregnancy test Excessive alcohol consumption (more than 30 g alcohol per day) or alcohol consumption within two days before sampling blood Disability to communicate with the investigator because of linguistic problems or because of a limited mental state Inclusion criteria for healthy subjects: Age matched with patients with anorexia nervosa (18 years to 30 years) Well status of health Female Negative pregnancy test No clinical relevant diagnosis, particularly no hyperlipoproteinemias (LDL \< 160 mg/dl) BMI \> 18,0 kg/m² and \< 25 kg/m²

Countries

Germany

Contacts

Primary ContactCaroline Storch
caroline.storch@med.uni-heidelberg.de0049 6221 56 39939

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026